ŠVÁBENSKÁ, Eva, David KOVÁŘ, Vít KRAJÍČEK, Jan PŘIBYL and Petr SKLÁDAL. Electrochemical Biosensor for Detection of Bioagents. International Journal of Electrochemical Science. Belgrade, Serbia: ESG, 2011, vol. 6, No 12, p. 5968-5979. ISSN 1452-3981.
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Basic information
Original name Electrochemical Biosensor for Detection of Bioagents
Name in Czech Elektrochemický biosensor pro detekci biologických látek
Authors ŠVÁBENSKÁ, Eva (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution), Vít KRAJÍČEK (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution).
Edition International Journal of Electrochemical Science, Belgrade, Serbia, ESG, 2011, 1452-3981.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.729
RIV identification code RIV/00216224:14310/11:00055984
Organization unit Faculty of Science
UT WoS 000297571400002
Keywords (in Czech) amerometrický sensor; sítotiskové elektrody; imunochemický detektor; imunosensor
Keywords in English amperometric sensor; screen-printed electrode; immunochemical detector; immunosensor
Tags AKR, rivok
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 16/4/2012 09:23.
Abstract
Simple and rapid detection and identification of dangerous bioagents is important for preventing illness or even death of people due to infectious diseases and bioterrorist threats. Electrochemical detection has a large potential for development of portable instrumentation suitable for analysis in field and especially combination with specificity of immunoassays seems promising. Initially, the construction of the immunosensing layer was realised and the effect of Au nanoparticles on the response of peroxidase was studied. An amperometric immunosensor was developed using screen-printed transducer, it employed specific capture of microbes in the sensing area by formation of an immunocomplex and its subsequent labelling using the tracer - antibody conjugated to peroxidase. The obtained sandwich electrochemical immunoassay was applied for the model microbial cells of B. subtilis. The total time of analysis was 20 min and the detection of cells below 104 CFU/ml was feasible. The presence of microbial cells at the sensing surface was confirmed using atomic force microscopy. The results appear promising for combination of the immunosensor with a cyclone sampler and analysis of Bacillus-based bioaerosols in field trials.
Links
LC06030, research and development projectName: Biomolekulární centrum
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre
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