ŠVÁBENSKÁ, Eva, David KOVÁŘ, Vít KRAJÍČEK, Jan PŘIBYL and Petr SKLÁDAL. Electrochemical Biosensor for Detection of Bioagents. International Journal of Electrochemical Science. Belgrade, Serbia: ESG, 2011, vol. 6, No 12, p. 5968-5979. ISSN 1452-3981. |
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@article{974916, author = {Švábenská, Eva and Kovář, David and Krajíček, Vít and Přibyl, Jan and Skládal, Petr}, article_location = {Belgrade, Serbia}, article_number = {12}, keywords = {amperometric sensor; screen-printed electrode; immunochemical detector; immunosensor}, language = {eng}, issn = {1452-3981}, journal = {International Journal of Electrochemical Science}, title = {Electrochemical Biosensor for Detection of Bioagents}, url = {http://www.electrochemsci.org/papers/vol6/6125968.pdf}, volume = {6}, year = {2011} }
TY - JOUR ID - 974916 AU - Švábenská, Eva - Kovář, David - Krajíček, Vít - Přibyl, Jan - Skládal, Petr PY - 2011 TI - Electrochemical Biosensor for Detection of Bioagents JF - International Journal of Electrochemical Science VL - 6 IS - 12 SP - 5968-5979 EP - 5968-5979 PB - ESG SN - 14523981 KW - amperometric sensor KW - screen-printed electrode KW - immunochemical detector KW - immunosensor UR - http://www.electrochemsci.org/papers/vol6/6125968.pdf L2 - http://www.electrochemsci.org/papers/vol6/6125968.pdf N2 - Simple and rapid detection and identification of dangerous bioagents is important for preventing illness or even death of people due to infectious diseases and bioterrorist threats. Electrochemical detection has a large potential for development of portable instrumentation suitable for analysis in field and especially combination with specificity of immunoassays seems promising. Initially, the construction of the immunosensing layer was realised and the effect of Au nanoparticles on the response of peroxidase was studied. An amperometric immunosensor was developed using screen-printed transducer, it employed specific capture of microbes in the sensing area by formation of an immunocomplex and its subsequent labelling using the tracer - antibody conjugated to peroxidase. The obtained sandwich electrochemical immunoassay was applied for the model microbial cells of B. subtilis. The total time of analysis was 20 min and the detection of cells below 104 CFU/ml was feasible. The presence of microbial cells at the sensing surface was confirmed using atomic force microscopy. The results appear promising for combination of the immunosensor with a cyclone sampler and analysis of Bacillus-based bioaerosols in field trials. ER -
ŠVÁBENSKÁ, Eva, David KOVÁŘ, Vít KRAJÍČEK, Jan PŘIBYL and Petr SKLÁDAL. Electrochemical Biosensor for Detection of Bioagents. \textit{International Journal of Electrochemical Science}. Belgrade, Serbia: ESG, 2011, vol.~6, No~12, p.~5968-5979. ISSN~1452-3981.
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