Electrochemical Biosensor for Detection of Bioagents

J 2011

Electrochemical Biosensor for Detection of Bioagents

ŠVÁBENSKÁ, Eva, David KOVÁŘ, Vít KRAJÍČEK, Jan PŘIBYL, Petr SKLÁDAL et. al.

Basic information

Original name

Electrochemical Biosensor for Detection of Bioagents

Name in Czech

Elektrochemický biosensor pro detekci biologických látek

Authors

ŠVÁBENSKÁ, Eva (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution), Vít KRAJÍČEK (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Electrochemical Science, Belgrade, Serbia, ESG, 2011, 1452-3981

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.729

RIV identification code

RIV/00216224:14310/11:00055984

Organization unit

Faculty of Science

UT WoS

000297571400002

Keywords (in Czech)

amerometrický sensor; sítotiskové elektrody; imunochemický detektor; imunosensor

Keywords in English

amperometric sensor; screen-printed electrode; immunochemical detector; immunosensor

Abstract

V originále

Simple and rapid detection and identification of dangerous bioagents is important for preventing illness or even death of people due to infectious diseases and bioterrorist threats. Electrochemical detection has a large potential for development of portable instrumentation suitable for analysis in field and especially combination with specificity of immunoassays seems promising. Initially, the construction of the immunosensing layer was realised and the effect of Au nanoparticles on the response of peroxidase was studied. An amperometric immunosensor was developed using screen-printed transducer, it employed specific capture of microbes in the sensing area by formation of an immunocomplex and its subsequent labelling using the tracer - antibody conjugated to peroxidase. The obtained sandwich electrochemical immunoassay was applied for the model microbial cells of B. subtilis. The total time of analysis was 20 min and the detection of cells below 104 CFU/ml was feasible. The presence of microbial cells at the sensing surface was confirmed using atomic force microscopy. The results appear promising for combination of the immunosensor with a cyclone sampler and analysis of Bacillus-based bioaerosols in field trials.

Links

LC06030, research and development project

Name: Biomolekulární centrum

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre

Citovat

ŠVÁBENSKÁ, Eva, David KOVÁŘ, Vít KRAJÍČEK, Jan PŘIBYL and Petr SKLÁDAL. Electrochemical Biosensor for Detection of Bioagents. International Journal of Electrochemical Science. Belgrade, Serbia: ESG, 2011, vol. 6, No 12, p. 5968-5979. ISSN 1452-3981.

@article{974916,
   author = {Švábenská, Eva and Kovář, David and Krajíček, Vít and Přibyl, Jan and Skládal, Petr},
   article_location = {Belgrade, Serbia},
   article_number = {12},
   keywords = {amperometric sensor; screen-printed electrode; immunochemical detector; immunosensor},
   language = {eng},
   issn = {1452-3981},
   journal = {International Journal of Electrochemical Science},
   title = {Electrochemical Biosensor for Detection of Bioagents},
   url = {http://www.electrochemsci.org/papers/vol6/6125968.pdf},
   volume = {6},
   year = {2011}
}

TY  - JOUR
ID  - 974916
AU  - Švábenská, Eva - Kovář, David - Krajíček, Vít - Přibyl, Jan - Skládal, Petr
PY  - 2011
TI  - Electrochemical Biosensor for Detection of Bioagents
JF  - International Journal of Electrochemical Science
VL  - 6
IS  - 12
SP  - 5968-5979
EP  - 5968-5979
PB  - ESG
SN  - 14523981
KW  - amperometric sensor
KW  - screen-printed electrode
KW  - immunochemical detector
KW  - immunosensor
UR  - http://www.electrochemsci.org/papers/vol6/6125968.pdf
L2  - http://www.electrochemsci.org/papers/vol6/6125968.pdf
N2  - Simple and rapid detection and identification of dangerous bioagents is important for preventing illness or even death of people due to infectious diseases and bioterrorist threats. Electrochemical detection has a large potential for development of portable instrumentation suitable for analysis in field and especially combination with specificity of immunoassays seems promising. Initially, the construction of the immunosensing layer was realised and the effect of Au nanoparticles on the response of peroxidase was studied. An amperometric immunosensor was developed using screen-printed transducer, it employed specific capture of microbes in the sensing area by formation of an immunocomplex and its subsequent labelling using the tracer - antibody conjugated to peroxidase. The obtained sandwich electrochemical immunoassay was applied for the model microbial cells of B. subtilis. The total time of analysis was 20 min and the detection of cells below 104 CFU/ml was feasible. The presence of microbial cells at the sensing surface was confirmed using atomic force microscopy. The results appear promising for combination of the immunosensor with a cyclone sampler and analysis of Bacillus-based bioaerosols in field trials.
ER  -

ŠVÁBENSKÁ, Eva, David KOVÁŘ, Vít KRAJÍČEK, Jan PŘIBYL and Petr SKLÁDAL. Electrochemical Biosensor for Detection of Bioagents. \textit{International Journal of Electrochemical Science}. Belgrade, Serbia: ESG, 2011, vol.~6, No~12, p.~5968-5979. ISSN~1452-3981.

Detailed Information on Publication Record