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@article{974964,
author = {Kučera, Jiří and Bouchal, Pavel and Černá, Hana and Potěšil, David and Janiczek, Oldřich and Zdráhal, Zbyněk and Mandl, Martin},
article_location = {DORDRECHT (NETHERLANDS)},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s10482-011-9670-2},
keywords = {Acidithiobacillus; Anaerobic sulfur oxidation; Ferric iron reduction; Proteomics; MS analysis},
language = {eng},
issn = {0003-6072},
journal = {ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY},
title = {Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS},
volume = {101},
year = {2012}
}
TY - JOUR
ID - 974964
AU - Kučera, Jiří - Bouchal, Pavel - Černá, Hana - Potěšil, David - Janiczek, Oldřich - Zdráhal, Zbyněk - Mandl, Martin
PY - 2012
TI - Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS
JF - ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
VL - 101
IS - 3
SP - 561-573
EP - 561-573
PB - Springer
SN - 00036072
KW - Acidithiobacillus
KW - Anaerobic sulfur oxidation
KW - Ferric iron reduction
KW - Proteomics
KW - MS analysis
N2 - Elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans was investigated. The apparent Michaelis constant for ferric iron was 18.6 mM. An absence of anaerobic ferric iron reduction ability was observed in bacteria maintained on elemental sulfur for an extended period of time. Upon transition from ferrous iron to elemental sulfur medium, the cells exhibited similar kinetic characteristics of ferric iron reduction under anaerobic conditions to those of cells that were originally maintained on ferrous iron. Nevertheless, a total loss of anaerobic ferric iron reduction ability after the sixth passage in elemental sulfur medium was demonstrated. The first proteomic screening of total cell lysates of anaerobically incubated bacteria resulted in the detection of 1599 protein spots in the master two-dimensional electrophoresis gel. A set of 59 more abundant and 49 less abundant protein spots that changed their protein abundances in an anaerobiosis-dependent manner was identified and compared to iron- and sulfur-grown cells, respectively. Proteomic analysis detected a significant increase in abundance under anoxic conditions of electron transporters, such as rusticyanin and cytochrome c552, involved in the ferrous iron oxidation pathway. Therefore we suggest the incorporation of rus-operon encoded proteins in the anaerobic respiration pathway. Two sulfur metabolism proteins were identified, pyridine nucleotide-disulfide oxidoreductase and sulfide-quinone reductase. The important transcription regulator, ferric uptake regulation protein, was anaerobically more abundant. The anaerobic expression of several proteins involved in cell envelope formation indicated a gradual adaptation to elemental sulfur oxidation.
ER -
KUČERA, Jiří, Pavel BOUCHAL, Hana ČERNÁ, David POTĚŠIL, Oldřich JANICZEK, Zbyněk ZDRÁHAL and Martin MANDL. Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS. \textit{ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY}. DORDRECHT (NETHERLANDS): Springer, 2012, vol.~101, No~3, p.~561-573. ISSN~0003-6072. doi:10.1007/s10482-011-9670-2.
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