KUČERA, Jiří, Pavel BOUCHAL, Hana ČERNÁ, David POTĚŠIL, Oldřich JANICZEK, Zbyněk ZDRÁHAL and Martin MANDL. Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY. DORDRECHT (NETHERLANDS): Springer, 2012, vol. 101, No 3, p. 561-573. ISSN 0003-6072. doi:10.1007/s10482-011-9670-2.
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Basic information
Original name Kinetics of anaerobic elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans and protein identification by comparative 2-DE-MS/MS
Name in Czech Kinetika anaerobní oxidace síry železitými ionty u bakterie Acidithiobacillus ferrooxidans a identifikace proteinů pomocí 2-DE-MS/MS
Authors KUČERA, Jiří (203 Czech Republic, belonging to the institution), Pavel BOUCHAL (203 Czech Republic, belonging to the institution), Hana ČERNÁ (203 Czech Republic, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Oldřich JANICZEK (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Martin MANDL (203 Czech Republic, guarantor, belonging to the institution).
Edition ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, DORDRECHT (NETHERLANDS), Springer, 2012, 0003-6072.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.072
RIV identification code RIV/00216224:14310/12:00057288
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1007/s10482-011-9670-2
UT WoS 000300314200010
Keywords (in Czech) Acidithiobacillus; Anaerobní oxidace síry, Redukce Fe(III), Proteomika; MS analýza
Keywords in English Acidithiobacillus; Anaerobic sulfur oxidation; Ferric iron reduction; Proteomics; MS analysis
Tags AKR, podil
Tags International impact, Reviewed
Changed by Changed by: doc. Ing. Martin Mandl, CSc., učo 1408. Changed: 13. 1. 2016 09:57.
Abstract
Elemental sulfur oxidation by ferric iron in Acidithiobacillus ferrooxidans was investigated. The apparent Michaelis constant for ferric iron was 18.6 mM. An absence of anaerobic ferric iron reduction ability was observed in bacteria maintained on elemental sulfur for an extended period of time. Upon transition from ferrous iron to elemental sulfur medium, the cells exhibited similar kinetic characteristics of ferric iron reduction under anaerobic conditions to those of cells that were originally maintained on ferrous iron. Nevertheless, a total loss of anaerobic ferric iron reduction ability after the sixth passage in elemental sulfur medium was demonstrated. The first proteomic screening of total cell lysates of anaerobically incubated bacteria resulted in the detection of 1599 protein spots in the master two-dimensional electrophoresis gel. A set of 59 more abundant and 49 less abundant protein spots that changed their protein abundances in an anaerobiosis-dependent manner was identified and compared to iron- and sulfur-grown cells, respectively. Proteomic analysis detected a significant increase in abundance under anoxic conditions of electron transporters, such as rusticyanin and cytochrome c552, involved in the ferrous iron oxidation pathway. Therefore we suggest the incorporation of rus-operon encoded proteins in the anaerobic respiration pathway. Two sulfur metabolism proteins were identified, pyridine nucleotide-disulfide oxidoreductase and sulfide-quinone reductase. The important transcription regulator, ferric uptake regulation protein, was anaerobically more abundant. The anaerobic expression of several proteins involved in cell envelope formation indicated a gradual adaptation to elemental sulfur oxidation.
Abstract (in Czech)
Byla studována kinetika a proteomika anaerobní oxidace síry ionty Fe(III) u Acidithiobacillus ferrooxidans. Zdánlivá Michaelisova konstanta pro Fe(III) byla 18,6 mM. Bakterie udržované na síře ztrácely schopnost anaerobní oxidace síry. První proteomická analýza buněčného lýzátu vyústila v 1599 proteinových spotů za použití srovnávací 2-DE. Proteinové složení bylo konfrontováno s bakteriemi oxidujícími železo(II) a síru a vyhodnoceny typické proteiny odpovídající anaerobióze. Výsledky poukazují na hlavní metabolické dráhy daného procesu.
Links
GA525/08/0697, research and development projectName: Aktivita acidofilních bakterií oxidujících síru za aerobních a anaerobních podmínek
Investor: Czech Science Foundation, Activity of acidophilic sulfur-oxidizing bacteria under aerobic and anaerobic conditions
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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