Whole Genome Sequences of Three Treponema pallidum ssp.
pertenue Strains: Yaws and Syphilis Treponemes Differ in Less
than 0.2% of the Genome Sequence

J 2012

Whole Genome Sequences of Three Treponema pallidum ssp. pertenue Strains: Yaws and Syphilis Treponemes Differ in Less than 0.2% of the Genome Sequence

ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Lei CHEN, Petra POSPÍŠILOVÁ, Michal STROUHAL et. al.

Basic information

Original name

Whole Genome Sequences of Three Treponema pallidum ssp. pertenue Strains: Yaws and Syphilis Treponemes Differ in Less than 0.2% of the Genome Sequence

Authors

ČEJKOVÁ, Darina (203 Czech Republic, belonging to the institution), Marie ZOBANÍKOVÁ (203 Czech Republic, belonging to the institution), Lei CHEN (840 United States of America), Petra POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Xiang QIN (840 United States of America), Lenka MIKALOVÁ (203 Czech Republic, belonging to the institution), Stephen J. NORRIS (840 United States of America), Donna M. MUZNY (840 United States of America), Richard A. GIBBS (840 United States of America), Lucinda L. FULTON (840 United States of America), Erica SODERGREN (840 United States of America), George M. WEINSTOCK (840 United States of America) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLoS Neglected Tropical Diseases, San Francisco, Public Library of Science, 2012, 1935-2727

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Art.No. e1471

Impact factor

Impact factor: 4.716 in 2011

RIV identification code

RIV/00216224:14110/12:00081800

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pntd.0001471

UT WoS

000300416100021

Keywords in English

Treponema pallidum; Syphilis; Genome; Yaws

Abstract

V originále

Spirochete Treponema pallidum ssp. pertenue (TPE) is the causative agent of yaws while strains of Treponema pallidum ssp. pallidum (TPA) cause syphilis. Both yaws and syphilis are distinguished on the basis of epidemiological characteristics and clinical symptoms. Neither treponeme can reproduce outside the host organism, which precludes the use of standard molecular biology techniques used to study cultivable pathogens. In this study, we determined high quality whole genome sequences of TPE strains and compared them to known genetic information for T. pallidum ssp. pallidum strains. The genome structure was identical in all three TPE strains and also between TPA and TPE strains. The TPE genome length ranged between 1,139,330 bp and 1,139,744 bp. The overall sequence identity between TPA and TPE genomes was 99.8%, indicating that the two pathogens are extremely closely related. A set of 34 TPE genes (3.5%) encoded proteins containing six or more amino acid replacements or other major sequence changes. These genes more often belonged to the group of genes with predicted virulence and unknown functions suggesting their involvement in infection differences between yaws and syphilis.

Links

GA310/07/0321, research and development project

Name: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Czech Science Foundation

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Lei CHEN, Petra POSPÍŠILOVÁ, Michal STROUHAL, Xiang QIN, Lenka MIKALOVÁ, Stephen J. NORRIS, Donna M. MUZNY, Richard A. GIBBS, Lucinda L. FULTON, Erica SODERGREN, George M. WEINSTOCK and David ŠMAJS. Whole Genome Sequences of Three Treponema pallidum ssp. pertenue Strains: Yaws and Syphilis Treponemes Differ in Less than 0.2% of the Genome Sequence. PLoS Neglected Tropical Diseases. San Francisco: Public Library of Science, 2012, vol. 6, No 1, p. "nestrankovano", 14 pp. ISSN 1935-2727. Available from: https://dx.doi.org/10.1371/journal.pntd.0001471.

@article{976503,
   author = {Čejková, Darina and Zobaníková, Marie and Chen, Lei and Pospíšilová, Petra and Strouhal, Michal and Qin, Xiang and Mikalová, Lenka and Norris, Stephen J. and Muzny, Donna M. and Gibbs, Richard A. and Fulton, Lucinda L. and Sodergren, Erica and Weinstock, George M. and Šmajs, David},
   article_location = {San Francisco},
   article_number = {1},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0001471},
   keywords = {Treponema pallidum; Syphilis; Genome; Yaws},
   language = {eng},
   issn = {1935-2727},
   journal = {PLoS Neglected Tropical Diseases},
   title = {Whole Genome Sequences of Three Treponema pallidum ssp. pertenue Strains: Yaws and Syphilis Treponemes Differ in Less than 0.2% of the Genome Sequence},
   url = {http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001471},
   volume = {6},
   year = {2012}
}

TY  - JOUR
ID  - 976503
AU  - Čejková, Darina - Zobaníková, Marie - Chen, Lei - Pospíšilová, Petra - Strouhal, Michal - Qin, Xiang - Mikalová, Lenka - Norris, Stephen J. - Muzny, Donna M. - Gibbs, Richard A. - Fulton, Lucinda L. - Sodergren, Erica - Weinstock, George M. - Šmajs, David
PY  - 2012
TI  - Whole Genome Sequences of Three Treponema pallidum ssp. pertenue Strains: Yaws and Syphilis Treponemes Differ in Less than 0.2% of the Genome Sequence
JF  - PLoS Neglected Tropical Diseases
VL  - 6
IS  - 1
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - Public Library of Science
SN  - 19352727
KW  - Treponema pallidum
KW  - Syphilis
KW  - Genome
KW  - Yaws
UR  - http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001471
L2  - http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001471
N2  - Spirochete Treponema pallidum ssp. pertenue (TPE) is the causative agent of yaws while strains of Treponema pallidum ssp. pallidum (TPA) cause syphilis. Both yaws and syphilis are distinguished on the basis of epidemiological characteristics and clinical symptoms. Neither treponeme can reproduce outside the host organism, which precludes the use of standard molecular biology techniques used to study cultivable pathogens. In this study, we determined high quality whole genome sequences of TPE strains and compared them to known genetic information for T. pallidum ssp. pallidum strains. The genome structure was identical in all three TPE strains and also between TPA and TPE strains. The TPE genome length ranged between 1,139,330 bp and 1,139,744 bp. The overall sequence identity between TPA and TPE genomes was 99.8%, indicating that the two pathogens are extremely closely related. A set of 34 TPE genes (3.5%) encoded proteins containing six or more amino acid replacements or other major sequence changes. These genes more often belonged to the group of genes with predicted virulence and unknown functions suggesting their involvement in infection differences between yaws and syphilis.
ER  -

ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Lei CHEN, Petra POSPÍŠILOVÁ, Michal STROUHAL, Xiang QIN, Lenka MIKALOVÁ, Stephen J. NORRIS, Donna M. MUZNY, Richard A. GIBBS, Lucinda L. FULTON, Erica SODERGREN, George M. WEINSTOCK and David ŠMAJS. Whole Genome Sequences of Three Treponema pallidum ssp. pertenue Strains: Yaws and Syphilis Treponemes Differ in Less than 0.2\%{} of the Genome Sequence. \textit{PLoS Neglected Tropical Diseases}. San Francisco: Public Library of Science, 2012, vol.~6, No~1, p.~''nestrankovano'', 14 pp. ISSN~1935-2727. Available from: https://dx.doi.org/10.1371/journal.pntd.0001471.

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