Metallothionein Electrochemically Determined using Brdicka
Reaction as a Promising Blood Marker of Head and Neck Malignant
Tumours

J 2012

Metallothionein Electrochemically Determined using Brdicka Reaction as a Promising Blood Marker of Head and Neck Malignant Tumours

KREJCOVA, Ludmila, Ivo FABRIK, David HYNEK, Soňa KRIZKOVA, Jaromír GUMULEC et. al.

Basic information

Original name

Metallothionein Electrochemically Determined using Brdicka Reaction as a Promising Blood Marker of Head and Neck Malignant Tumours

Authors

KREJCOVA, Ludmila (203 Czech Republic, guarantor), Ivo FABRIK (203 Czech Republic), David HYNEK (203 Czech Republic), Soňa KRIZKOVA (203 Czech Republic), Jaromír GUMULEC (203 Czech Republic, belonging to the institution), Marketa RYVOLOVA (203 Czech Republic), Vojtech ADAM (203 Czech Republic), Petr BABULA (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution), Marie STIBOROVA (203 Czech Republic), Jaromir HUBALEK (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Hana BINKOVÁ (203 Czech Republic, belonging to the institution), Tomáš ECKSCHLAGER (203 Czech Republic) and René KIZEK (203 Czech Republic)

Edition

International Journal of Electrochemical Science, 2012, 1452-3981

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Serbia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.729 in 2011

RIV identification code

RIV/00216224:14110/12:00059527

Organization unit

Faculty of Medicine

UT WoS

000302730300003

Keywords in English

electrochemical detection; head and neck cancer; voltammetry; catalytic signal; metallothionein; marker

Abstract

V originále

There are more than half of million patients who fall ill with head and neck carcinoma per year, which 6 % of annually newly diagnosed malignant diseases in the world. Group of head and neck tumours includes tumours occurring in the upper respiratory tract and upper digestive tract. They include tumours occurring in the area of oral cavity, tumours of hard and soft palate, gingivae and tongue. However, suitable markers in the case of head and neck carcinoma have not been satisfactory identified. Metallothioneins as a group of proteins with unknown but important role in development of a tumour can be assumed as a potential marker. Therefore, electrochemical monitoring of metallothionein levels in patients suffering from primary malignant tumour in head and neck area and examination of a suitability of electrochemical detection as a technique to be used in clinical practise are the most important aims of this study. Differential pulse voltammetry Brdicka reaction was optimized (time of accumulation 240 s, dilution of a sample 100 times and sample injection 10 microl) and used for analysis off blood obtained from 145 patients with newly diagnosed malignant tumour disease in the head and neck area. As a control, fifty eight blood samples were used. The obtained data enabled us to suggest reference MT level in blood of healthy human within the interval from 0.2 to 0.8 microM. In the tumour blood samples, the most extend group was represented by patients suffering from oropharyngeal cancer (n = 69, 47.5 %), laryngeal cancer (n = 36, 24.8 %), hypopharyngeal cancer (n = 14, 9.6 %), oral cavity cancer (n = 18, 12.4 %) and rarely occurring nasal cavity and paranasal sinus cancer (n = 4, 2.7 %) and parotid carcinoma (n = 4, 2.7%). Age median of the studied group was 60 years. Determined MT levels in blood of patients varied from 1.08 to 6.39 microM, whereas average values differed in the accordance with tumour localization. Differences between individual localizations are discussed. In conclusion, MT levels are closely associated with the rate of tumour differentiation, stage of tumour disease and tumour cell characteristics. Our study demonstrates not only changes in blood MT levels in patients suffering from malignant tumour disease in head and neck area, but also suitability of electrochemical techniques for blood analysis.

Citovat

KREJCOVA, Ludmila, Ivo FABRIK, David HYNEK, Soňa KRIZKOVA, Jaromír GUMULEC, Marketa RYVOLOVA, Vojtech ADAM, Petr BABULA, Libuše TRNKOVÁ, Marie STIBOROVA, Jaromir HUBALEK, Michal MASAŘÍK, Hana BINKOVÁ, Tomáš ECKSCHLAGER and René KIZEK. Metallothionein Electrochemically Determined using Brdicka Reaction as a Promising Blood Marker of Head and Neck Malignant Tumours. International Journal of Electrochemical Science. 2012, vol. 7, No 3, p. 1767 - 1784. ISSN 1452-3981.

@article{976690,
   author = {Krejcova, Ludmila and Fabrik, Ivo and Hynek, David and Krizkova, Soňa and Gumulec, Jaromír and Ryvolova, Marketa and Adam, Vojtech and Babula, Petr and Trnková, Libuše and Stiborova, Marie and Hubalek, Jaromir and Masařík, Michal and Binková, Hana and Eckschlager, Tomáš and Kizek, René},
   article_number = {3},
   keywords = {electrochemical detection; head and neck cancer; voltammetry; catalytic signal; metallothionein; marker},
   language = {eng},
   issn = {1452-3981},
   journal = {International Journal of Electrochemical Science},
   title = {Metallothionein Electrochemically Determined using Brdicka Reaction as a Promising Blood Marker of Head and Neck Malignant Tumours},
   volume = {7},
   year = {2012}
}

TY  - JOUR
ID  - 976690
AU  - Krejcova, Ludmila - Fabrik, Ivo - Hynek, David - Krizkova, Soňa - Gumulec, Jaromír - Ryvolova, Marketa - Adam, Vojtech - Babula, Petr - Trnková, Libuše - Stiborova, Marie - Hubalek, Jaromir - Masařík, Michal - Binková, Hana - Eckschlager, Tomáš - Kizek, René
PY  - 2012
TI  - Metallothionein Electrochemically Determined using Brdicka Reaction as a Promising Blood Marker of Head and Neck Malignant Tumours
JF  - International Journal of Electrochemical Science
VL  - 7
IS  - 3
SP  - 1767 - 1784
EP  - 1767 - 1784
SN  - 14523981
KW  - electrochemical detection
KW  - head and neck cancer
KW  - voltammetry
KW  - catalytic signal
KW  - metallothionein
KW  - marker
N2  - There are more than half of million patients who fall ill with head and neck carcinoma per year, which 6 % of annually newly diagnosed malignant diseases in the world. Group of head and neck tumours includes tumours occurring in the upper respiratory tract and upper digestive tract. They include tumours occurring in the area of oral cavity, tumours of hard and soft palate, gingivae and tongue. However, suitable markers in the case of head and neck carcinoma have not been satisfactory identified. Metallothioneins as a group of proteins with unknown but important role in development of a tumour can be assumed as a potential marker. Therefore, electrochemical monitoring of metallothionein levels in patients suffering from primary malignant tumour in head and neck area and examination of a suitability of electrochemical detection as a technique to be used in clinical practise are the most important aims of this study. Differential pulse voltammetry Brdicka reaction was optimized (time of accumulation 240 s, dilution of a sample 100 times and sample injection 10 microl) and used for analysis off blood obtained from 145 patients with newly diagnosed malignant tumour disease in the head and neck area. As a control, fifty eight blood samples were used. The obtained data enabled us to suggest reference MT level in blood of healthy human within the interval from 0.2 to 0.8 microM. In the tumour blood samples, the most extend group was represented by patients suffering from oropharyngeal cancer (n = 69, 47.5 %), laryngeal cancer (n = 36, 24.8 %), hypopharyngeal cancer (n = 14, 9.6 %), oral cavity cancer (n = 18, 12.4 %) and rarely occurring nasal cavity and paranasal sinus cancer (n = 4, 2.7 %) and parotid carcinoma (n = 4, 2.7%). Age median of the studied group was 60 years. Determined MT levels in blood of patients varied from 1.08 to 6.39 microM, whereas average values differed in the accordance with tumour localization. Differences between individual localizations are discussed. In conclusion, MT levels are closely associated with the rate of tumour differentiation, stage of tumour disease and tumour cell characteristics. Our study demonstrates not only changes in blood MT levels in patients suffering from malignant tumour disease in head and neck area, but also suitability of electrochemical techniques for blood analysis.
ER  -

KREJCOVA, Ludmila, Ivo FABRIK, David HYNEK, Soňa KRIZKOVA, Jaromír GUMULEC, Marketa RYVOLOVA, Vojtech ADAM, Petr BABULA, Libuše TRNKOVÁ, Marie STIBOROVA, Jaromir HUBALEK, Michal MASAŘÍK, Hana BINKOVÁ, Tomáš ECKSCHLAGER and René KIZEK. Metallothionein Electrochemically Determined using Brdicka Reaction as a Promising Blood Marker of Head and Neck Malignant Tumours. \textit{International Journal of Electrochemical Science}. 2012, vol.~7, No~3, p.~1767 - 1784. ISSN~1452-3981.

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