c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis

KNOPFOVÁ, Lucia, Petr BENEŠ, Lucie PEKARČÍKOVÁ, Markéta HERMANOVÁ, Michal MASAŘÍK, Zuzana PERNICOVÁ, Karel SOUČEK and Jan ŠMARDA. c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis. Molecular Cancer. BioMed Central Ltd, 2012, vol. 11, MARCH, p. 15-29. ISSN 1476-4598. Available from: https://dx.doi.org/10.1186/1476-4598-11-15.

Basic information

• Original name: c-Myb regulates matrix metalloproteinases 1/9, and cathepsin D: implications for matrix-dependent breast cancer cell invasion and metastasis
• Name in Czech: Regulace matrixovým metraloproteináz 1/9 a katepsinu D proteinem c-Myb: implikace pro matrix-dependentní ivazivitu a metastázování nádoru prsu
• Authors: KNOPFOVÁ, Lucia (203 Czech Republic, belonging to the institution), Petr BENEŠ (203 Czech Republic, belonging to the institution), Lucie PEKARČÍKOVÁ (203 Czech Republic, belonging to the institution), Markéta HERMANOVÁ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic), Zuzana PERNICOVÁ (203 Czech Republic), Karel SOUČEK (203 Czech Republic) and Jan ŠMARDA (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Molecular Cancer, BioMed Central Ltd, 2012, 1476-4598.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 5.134
• RIV identification code: RIV/00216224:14310/12:00057318
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1186/1476-4598-11-15
• UT WoS: 000302805400001
• Keywords (in Czech): c-Myb; metastáza; rakovina prsu; matrixová metaloproteináza; katepsin D; mimobuněčná matrix
• Keywords in English: c-Myb; Metastasis; Breast cancer; Matrix metalloproteinase; Cathepsin D; Extracellular matrix
• Tags: AKR, rivok
• Tags: International impact, Reviewed

Abstract

The aim of this study was to investigate the role of c-Myb in the metastatic spread of breast tumors. We ectopically expressed c-Myb in human MDA-MB-231 and mouse 4T1 mammary cancer cells to find that c-Myb enhanced migration and ability of these cells to invade Matrigel but not the collagen I matrix in vitro. c-Myb strongly increased the expression/activity of cathepsin D and matrix metalloproteinase (MMP) 9 and significantly downregulated MMP1. The gene coding for cathepsin D was found to act as the c-Myb-responsive gene and downstream effector of the migration-promoting function of c-Myb. Finally, we demonstrated that c-Myb delayed the growth of mammary tumors in BALB/c mice and affected the metastatic potential of breast cancer cells in an organ-specific manner. This study identified c-Myb as a matrix-dependent regulator of invasive behavior of breast cancer cells.

Links

• GA301/09/1115, research and development project: Name: Úloha proteinů c-Myb a Cox-2 při tvorbě střevních nádorů

Investor: Czech Science Foundation, The role of c-Myb and Cox-2 proteins in colon carcinogenesis

• GD204/08/H054, research and development project: Name: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• MUNI/C/0968/2010, interní kód MU: Name: Úloha proteinu c-Myb v regulaci migrace, invazivity a apoptózy buněk střevního karcinomu (Acronym: c-Myb a invazivita CT26)

Investor: Masaryk University, Rector's Program