The simultaneous determination of different posttranslational modifications of proteins – A comparison of approaches

DYČKA, Filip, Richard ČMELÍK and Janette BOBÁLOVÁ. The simultaneous determination of different posttranslational modifications of proteins – A comparison of approaches. In 29th Informal Meeting on Mass Spectrometry. 2011. ISBN 978-88-89884-19-5.

Basic information

• Original name: The simultaneous determination of different posttranslational modifications of proteins – A comparison of approaches
• Authors: DYČKA, Filip, Richard ČMELÍK and Janette BOBÁLOVÁ.
• Edition: 29th Informal Meeting on Mass Spectrometry, 2011.

Other information

• Type of outcome: Conference abstract
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• ISBN: 978-88-89884-19-5
• Keywords in English: posttranslational modifications ; IMAC ; titanium dioxide

Abstract

This work reports enrichment techniques of the phosphopeptides and peptides containing cysteine sulfonic acid residue using IMAC tips containing iron ions and titanium dioxide chromatography. The methods were optimized using tryptic peptides of alpha-casein and albumin. After enrichment procedure the modified peptides were analyzed by MALDI-TOF.

Links

• AV0Z40310501, plan (intention): Name: Moderní analytické techniky pro bioanalýzu, ekologii a nanotechnologie
• IAA600040701, research and development project: Name: Dynamika proteomu v odpovědi na zvýšené hladiny cytokininů u Arabidopsis

Investor: Academy of Sciences of the Czech Republic, Proteome dynamics in response to increased cytokinin levels in Arabidopsis

• 1M0570, research and development project: Name: Výzkumné centrum pro studium obsahových látek ječmene a chmele