Accelerated enzymatic digestion for the analysis of plant proteins

DYČKA, Filip, Pavel BOBÁĽ and Janette BOBÁLOVÁ. Accelerated enzymatic digestion for the analysis of plant proteins. 2011.

Basic information

• Original name: Accelerated enzymatic digestion for the analysis of plant proteins
• Authors: DYČKA, Filip, Pavel BOBÁĽ and Janette BOBÁLOVÁ.
• Edition: 2011.

Other information

• Type of outcome: Conference abstract
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• Keywords in English: ultrasonic ; infrared ; digestion

Abstract

The aim of this work was to demonstrate the usefulness of the fast methods using ultrasonic or infrared assisted in-gel and in-solution digestion for analysis of proteins extracted from plant material. Both alternative methodologies led to better results compared to the standard protocol.

Links

• AV0Z40310501, plan (intention): Name: Moderní analytické techniky pro bioanalýzu, ekologii a nanotechnologie
• IAA600040701, research and development project: Name: Dynamika proteomu v odpovědi na zvýšené hladiny cytokininů u Arabidopsis

Investor: Academy of Sciences of the Czech Republic, Proteome dynamics in response to increased cytokinin levels in Arabidopsis

• 1M0570, research and development project: Name: Výzkumné centrum pro studium obsahových látek ječmene a chmele
• 1M06030, research and development project: Name: Funkční genomika a proteomika ve šlechtění rostlin