SKLÁDAL, Petr, Eva ŠVÁBENSKÁ, Jiří ŽERAVÍK, Jan PŘIBYL, Petra ŠIŠKOVÁ, Torbjorn TJARNHAGE and Inga GUSTAFSON. Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols. Electroanalysis. WILEY-VCH Verlag GmbH, 2012, vol. 24, No 3, p. 539-546. ISSN 1040-0397. Available from: https://dx.doi.org/10.1002/elan.201100448.
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Basic information
Original name Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols
Authors SKLÁDAL, Petr (203 Czech Republic, guarantor, belonging to the institution), Eva ŠVÁBENSKÁ (203 Czech Republic, belonging to the institution), Jiří ŽERAVÍK (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Petra ŠIŠKOVÁ (203 Czech Republic, belonging to the institution), Torbjorn TJARNHAGE (752 Sweden) and Inga GUSTAFSON (752 Sweden).
Edition Electroanalysis, WILEY-VCH Verlag GmbH, 2012, 1040-0397.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.817
RIV identification code RIV/00216224:14310/12:00059725
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1002/elan.201100448
UT WoS 000300931000011
Keywords (in Czech) bioagen; sendvičové imunostanovení; biosensor; vzdálené měření; aerosolová komora
Keywords in English Bioagents; Sandwich immunoassay; Biosensors; Remote sensing; Aerosol chamber
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 9/4/2013 21:11.
Abstract
The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5a in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.
Abstract (in Czech)
Přenosný imunosensor ImmunoSMART byl ve spojení s cyklonem použit pro imunochemické stanovení bioaerosolu z Escherichia coli DH5alfa v aerosolové komoře.
Links
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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