a 2012

Aspergillus fumigatus lectin - a model system for the study of multivalent lectins

KOMÁREK, Jan, Josef HOUSER, Marie POKORNÁ, Nikola KOSTLÁNOVÁ, Anne IMBERTY et. al.

Basic information

Original name

Aspergillus fumigatus lectin - a model system for the study of multivalent lectins

Authors

KOMÁREK, Jan (203 Czech Republic), Josef HOUSER (203 Czech Republic), Marie POKORNÁ (203 Czech Republic), Nikola KOSTLÁNOVÁ (203 Czech Republic), Anne IMBERTY (250 France) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Struktura a interakce biomolekul II, 2012

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/12:00057369

Organization unit

Faculty of Science

Keywords (in Czech)

Aspergillus fumigatus; lektiny

Keywords in English

Aspergillus fumigatus; lectins
Změněno: 26/4/2012 13:39, Mgr. Jan Komárek, Ph.D.

Abstract

V originále

Lectins are sugar-specific proteins involved in recognition events in a variety of physiological and pathological processes. The project is focused on a detailed characterization of L-fucose specific lectin AFL from ascomycete Aspergillus fumigatus. Structure-function studies of AFL revealed presence of six binding sites with different affinities and specificities towards fucosylated oligosaccharides. Detailed knowledge of differences in binding properties of individual AFL binding sites would be important in understanding the basis of protein-carbohydrate recognition. Further study is therefore needed to examine the fine details in behaviour of different AFL binding sites. This study uses a novel approach which is based on a synthetic gene and site-directed mutagenesis. The procedure should result in preparation of six monovalent forms of AFL (corresponding to individual binding sites) and their characterization from structure-function point of view.

Links

GD301/09/H004, research and development project
Name: Molekulární a strukturní biologie vybraných cytostatik. Od mechanistických studií k chemoterapii rakoviny
Investor: Czech Science Foundation