HOLUB, Peter, Jana LALÁKOVÁ, Hana ČERNÁ, Josef PASULKA, Marie SÁRAZOVÁ, Kristýna HRAZDILOVÁ, Maria SAŇUDO ARCE, Fruzsina HÓBOR, Richard ŠTEFL and Štěpánka VAŇÁČOVÁ. Air2p is critical for the assembly and RNA-binding of the TRAMP complex and the KOW domain of Mtr4p is crucial for exosome activation. Nucleic Acids Research. Oxford: Oxford University Press, 2012, vol. 40, No 12, p. 5679-5693. ISSN 0305-1048. doi:10.1093/nar/gks223.
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Basic information
Original name Air2p is critical for the assembly and RNA-binding of the TRAMP complex and the KOW domain of Mtr4p is crucial for exosome activation
Authors HOLUB, Peter (203 Czech Republic, belonging to the institution), Jana LALÁKOVÁ (703 Slovakia, belonging to the institution), Hana ČERNÁ (203 Czech Republic, belonging to the institution), Josef PASULKA (203 Czech Republic, belonging to the institution), Marie SÁRAZOVÁ (203 Czech Republic, belonging to the institution), Kristýna HRAZDILOVÁ (203 Czech Republic, belonging to the institution), Maria SAŇUDO ARCE (724 Spain, belonging to the institution), Fruzsina HÓBOR (348 Hungary, belonging to the institution), Richard ŠTEFL (203 Czech Republic, belonging to the institution) and Štěpánka VAŇÁČOVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition Nucleic Acids Research, Oxford, Oxford University Press, 2012, 0305-1048.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 8.278
RIV identification code RIV/00216224:14740/12:00057391
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1093/nar/gks223
UT WoS 000305829000049
Keywords in English RNA recognition; binding; RNA processing; RNA-protein interaction
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 7. 4. 2013 12:25.
Abstract
Trf4/5p-Air1/2p-Mtr4p polyadenylation complex (TRAMP) is an essential component of nuclear RNA surveillance in yeast. It recognizes a variety of nuclear transcripts produced by all three RNA polymerases, adds short poly(A) tails to aberrant or unstable RNAs and activates the exosome for their degradation. Despite the advances in understanding the structural features of the isolated complex subunits or their fragments, the details of complex assembly, RNA recognition and exosome activation remain poorly understood. Here we provide the first understanding of the RNA binding mode of the complex. We show that Air2p is an RNA-binding subunit of TRAMP. We identify the zinc knuckles (ZnK) 2, 3 and 4 as the RNA-binding domains, and reveal the essentiality of ZnK4 for TRAMP4 polyadenylation activity. Furthermore, we identify Air2p as the key component of TRAMP4 assembly providing bridging between Mtr4p and Trf4p. The former is bound via the N-terminus of Air2p, while the latter is bound via ZnK5, the linker between ZnK4 and 5 and the C-terminus of the protein. Finally, we uncover the RNA binding part of the Mtr4p arch, the KOW domain, as the essential component for TRAMP-mediated exosome activation.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GAP305/10/1490, research and development projectName: Strukturní podstata ukončení transkripce nezávislé na poly(A) signálu
Investor: Czech Science Foundation
GAP305/11/1095, research and development projectName: Funkční a biochemická charakterizace proteinu Dis3L2, třetího lidského homologu hlavní kvasinkové exosomové nukleázy Dis3p
Investor: Czech Science Foundation, Functional and biochemical characterization of Dis3L2, the third mammalian homolog of the key yeast exosome nuclease Dis3p
GBP305/12/G034, research and development projectName: Centrum biologie RNA
084316/Z/07/Z, interní kód MUName: Polyadenylation and mechanisms of nuclear RNA quality control
Investor: Wellcome Trust, Polyadenylation and mechanisms of nuclear RNA quality control
1642, interní kód MUName: EMBO Young Investigator Programme (Acronym: EMBO)
Investor: EMBO (European Molecular Biology Organization), EMBO Young Investigator Programme
205872, interní kód MUName: Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions (Acronym: POSTBIOMIN)
Investor: European Union, Program developing interdisciplinary research POtential for the STudies of BIOMolecular INteractions, Capacities
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