NECHVÁTALOVÁ, Jana, Zdenka PIKULOVÁ, Dagmar STIKAROVSKÁ, Sáva PEŠÁK, Marcela VLKOVÁ and Jiří LITZMAN. B-lymphocyte Subpopulations in Patients with Selective IgA Deficiency. Journal of Clinical Immunology. 2012, vol. 32, No 3, p. 441-448. ISSN 0271-9142. doi:10.1007/s10875-012-9655-6.
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Basic information
Original name B-lymphocyte Subpopulations in Patients with Selective IgA Deficiency
Authors NECHVÁTALOVÁ, Jana (203 Czech Republic, guarantor, belonging to the institution), Zdenka PIKULOVÁ (203 Czech Republic), Dagmar STIKAROVSKÁ (203 Czech Republic), Sáva PEŠÁK (203 Czech Republic, belonging to the institution), Marcela VLKOVÁ (203 Czech Republic, belonging to the institution) and Jiří LITZMAN (203 Czech Republic, belonging to the institution).
Edition Journal of Clinical Immunology, 2012, 0271-9142.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30300 3.3 Health sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.382
RIV identification code RIV/00216224:14110/12:00059958
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1007/s10875-012-9655-6
UT WoS 000305982100005
Keywords in English IgA deficiency . common variable immunodeficiency . B-lymphocyte subpopulations
Tags International impact
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 3/4/2013 11:53.
Abstract
Selective deficiency IgA (IgAD) is the most common primary abnormality of immunoglobulin production with unknown pathophysiology. It is genetically related to common variable immunodeficiency (CVID), where besides IgA also IgG and frequently IgM serum levels are decreased. In this study we focused on determination of B-lymphocyte developmental stages and searching for similarities between CVID and IgAD. Materials and Methods Using flow cytometry we determined major lymphocyte subpopulations and Blymphocyte subsets: naive (CD27-IgD+), marginal zone cells (CD27+IgD+), class-switched memory cells (CD27+IgD-), “double-negative” B cells (CD27-IgD-), transitional cells (IgM++CD38++), plasmablasts (CD38+++IgM+ or IgM-), and CD21lowCD38low cells in 80 patients with IgAD, 48 patients with CVID, and 80 control persons. Results Compared to healthy controls, a decrease in the absolute number and frequency of CD4+ cells (both<0.001) was observed in IgAD patients. A decrease in the frequency of switched memory cells (P<0.001), transitional cells (P00.035) as well as plasmablasts (P<0.001) and an increase in the CD21lowCD38low subset (P00.007) was observed in IgAD patients compared to control persons. No significant differences were observed in the remaining B-cell developmental subsets. A decrease in CD27+IgD- (<0.4% of peripheral blood lymphocytes), frequently observed in CVID patients and also previously reported in IgAD, was found in only five patients (6%) with IgAD, two of them being firstdegree relatives of CVID patients. Conclusion Our results show a decrease of terminally differentiated B-lymphocyte subsets in patients with IgAD, similar as previously found in patients with CVID, but these results are less expressed than in CVID patients.
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