| CERNEI, Natalia, Ondřej ZITKA, Markéta RYVOLOVÁ, Vojtěch ADAM, Michal MASAŘÍK, Jaromír HUBÁLEK and René KIZEK. Spectrometric and Electrochemical Analysis of Sarcosine as a Potential Prostate Carcinoma Marker. International Journal of Electrochemical Science. 2012, vol. 7, No 5, p. 4286-4301. ISSN 1452-3981. |
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@article{982602,
author = {Cernei, Natalia and Zitka, Ondřej and Ryvolová, Markéta and Adam, Vojtěch and Masařík, Michal and Hubálek, Jaromír and Kizek, René},
article_number = {5},
keywords = {Metabolomics; Cancer marker; Ninhydrin; UV-VIS detection; Ionic separation; Electrochemical detection; Carbon porous electrode},
language = {eng},
issn = {1452-3981},
journal = {International Journal of Electrochemical Science},
title = {Spectrometric and Electrochemical Analysis of Sarcosine as a Potential Prostate Carcinoma Marker},
volume = {7},
year = {2012}
}
TY - JOUR
ID - 982602
AU - Cernei, Natalia - Zitka, Ondřej - Ryvolová, Markéta - Adam, Vojtěch - Masařík, Michal - Hubálek, Jaromír - Kizek, René
PY - 2012
TI - Spectrometric and Electrochemical Analysis of Sarcosine as a Potential Prostate Carcinoma Marker
JF - International Journal of Electrochemical Science
VL - 7
IS - 5
SP - 4286-4301
EP - 4286-4301
SN - 14523981
KW - Metabolomics
KW - Cancer marker
KW - Ninhydrin
KW - UV-VIS detection
KW - Ionic separation
KW - Electrochemical detection
KW - Carbon porous electrode
N2 - Sarcosine, also known as N-methylglycine, is a natural ubiquitous non/protein amino acid which occurs as intermediate and side product in glycine synthesis and degradation. Recently, sarcosine has been investigated as a new putative marker in relation to prostate cancer. Sarcosine was identified as a differential metabolite that was greatly increased during prostate cancer progression to metastasis and could be detected in urine. It was also shown, that sarcosine addition to benign prostate cell cultures caused increase of their invasivity and motility. The aim of our study was to propose a low-cost, robust and simple method suitable for sarcosine determination in biological samples such as urine or blood plasma. For this purpose an ion exchange liquid chromatography as robust separation method was tested however this method suffers from insufficient limit of detection at the level of 70 microM of sarcosine. For more sensitive detection we optimized the off-line approach to ninhydrin derivatization of collected fractions. The fractions were collected and after addition of ninhydrin incubation of the mixture under the optimised temperature and time were done. Analysis of mixture was carried out by the simple UV-VIS spectrometer. The obtained limit of detection for this optimised procedure was promising 1.7 microM of sarcosine but this value is similar to physiologically occurring concentration of sarcosine in prostate cancer patients. Therefore we optimized the electrochemical method based on analysis of collected fractions by coulometric detection integrated as FIA-ED. Here we obtained the limits of detection of 110 nM of sarcosine, which is more than satisfactory for its determination in various matrixes such as urine or plasma of cancer patients.
ER -
CERNEI, Natalia, Ondřej ZITKA, Markéta RYVOLOVÁ, Vojtěch ADAM, Michal MASAŘÍK, Jaromír HUBÁLEK and René KIZEK. Spectrometric and Electrochemical Analysis of Sarcosine as a Potential Prostate Carcinoma Marker. \textit{International Journal of Electrochemical Science}. 2012, vol.~7, No~5, p.~4286-4301. ISSN~1452-3981.
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