New role for L-arginine in regulation of inducible
nitric-oxide-synthase-derived superoxide anion production in
raw 264.7 macrophages.

J 2011

New role for L-arginine in regulation of inducible nitric-oxide-synthase-derived superoxide anion production in raw 264.7 macrophages.

PEKAROVÁ, Michaela, Antonín LOJEK, Hana MARTIŠKOVÁ, Ondřej VAŠÍČEK, Lucia BINÓ et. al.

Basic information

Original name

New role for L-arginine in regulation of inducible nitric-oxide-synthase-derived superoxide anion production in raw 264.7 macrophages.

Authors

PEKAROVÁ, Michaela (703 Slovakia), Antonín LOJEK (203 Czech Republic), Hana MARTIŠKOVÁ (203 Czech Republic, belonging to the institution), Ondřej VAŠÍČEK (203 Czech Republic), Lucia BINÓ (703 Slovakia), Anna KLINKE (276 Germany), Denise LAU (276 Germany), Radek KUCHTA (203 Czech Republic), Jaroslav KADLEC (203 Czech Republic), Radimír VRBA (203 Czech Republic) and Lukáš KUBALA (203 Czech Republic)

Edition

The Scientific World Journal, NY, USA, 2011, 1537-744X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.524 in 2010

RIV identification code

RIV/00216224:14310/11:00081807

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1100/2011/321979

UT WoS

000298416400011

Keywords in English

Macrophages; L-arginine; inducible nitric oxide synthase; superoxide anion; NO

Abstract

V originále

Dietary supplementation with L-arginine was shown to improve immune responses in various inflammatory models. However, the molecular mechanisms underlying L-arginine effects on immune cells remain unrecognized. Herein, we tested the hypothesis that a limitation of L-arginine could lead to the uncoupled state of murine macrophage inducible nitric oxide synthase and, therefore, increase inducible nitric-oxide-synthase-derived superoxide anion formation. Importantly, we demonstrated that L-arginine dose- and time dependently potentiated superoxide anion production in bacterial endotoxin-stimulated macrophages, although it did not influence NADPH oxidase expression and activity.

Citovat

PEKAROVÁ, Michaela, Antonín LOJEK, Hana MARTIŠKOVÁ, Ondřej VAŠÍČEK, Lucia BINÓ, Anna KLINKE, Denise LAU, Radek KUCHTA, Jaroslav KADLEC, Radimír VRBA and Lukáš KUBALA. New role for L-arginine in regulation of inducible nitric-oxide-synthase-derived superoxide anion production in raw 264.7 macrophages. The Scientific World Journal. NY, USA, 2011, vol. 11, No 11, p. 2443-2457. ISSN 1537-744X. Available from: https://dx.doi.org/10.1100/2011/321979.

@article{983204,
   author = {Pekarová, Michaela and Lojek, Antonín and Martišková, Hana and Vašíček, Ondřej and Binó, Lucia and Klinke, Anna and Lau, Denise and Kuchta, Radek and Kadlec, Jaroslav and Vrba, Radimír and Kubala, Lukáš},
   article_location = {NY, USA},
   article_number = {11},
   doi = {http://dx.doi.org/10.1100/2011/321979},
   keywords = {Macrophages; L-arginine; inducible nitric oxide synthase; superoxide anion; NO},
   language = {eng},
   issn = {1537-744X},
   journal = {The Scientific World Journal},
   title = {New role for L-arginine in regulation of inducible nitric-oxide-synthase-derived superoxide anion production in raw 264.7 macrophages.},
   url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246759/?tool=pubmed},
   volume = {11},
   year = {2011}
}

TY  - JOUR
ID  - 983204
AU  - Pekarová, Michaela - Lojek, Antonín - Martišková, Hana - Vašíček, Ondřej - Binó, Lucia - Klinke, Anna - Lau, Denise - Kuchta, Radek - Kadlec, Jaroslav - Vrba, Radimír - Kubala, Lukáš
PY  - 2011
TI  - New role for L-arginine in regulation of inducible nitric-oxide-synthase-derived superoxide anion production in raw 264.7 macrophages.
JF  - The Scientific World Journal
VL  - 11
IS  - 11
SP  - 2443-2457
EP  - 2443-2457
SN  - 1537744X
KW  - Macrophages
KW  - L-arginine
KW  - inducible nitric oxide synthase
KW  - superoxide anion
KW  - NO
UR  - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246759/?tool=pubmed
N2  - Dietary supplementation with L-arginine was shown to improve immune responses in various inflammatory models. However, the molecular mechanisms underlying L-arginine effects on immune cells remain unrecognized. Herein, we tested the hypothesis that a limitation of L-arginine could lead to the uncoupled state of murine macrophage inducible nitric oxide synthase and, therefore, increase inducible nitric-oxide-synthase-derived superoxide anion formation. Importantly, we demonstrated that L-arginine dose- and time dependently potentiated superoxide anion production in bacterial endotoxin-stimulated macrophages, although it did not influence NADPH oxidase expression and activity.
ER  -

PEKAROVÁ, Michaela, Antonín LOJEK, Hana MARTIŠKOVÁ, Ondřej VAŠÍČEK, Lucia BINÓ, Anna KLINKE, Denise LAU, Radek KUCHTA, Jaroslav KADLEC, Radimír VRBA and Lukáš KUBALA. New role for L-arginine in regulation of inducible nitric-oxide-synthase-derived superoxide anion production in raw 264.7 macrophages. \textit{The Scientific World Journal}. NY, USA, 2011, vol.~11, No~11, p.~2443-2457. ISSN~1537-744X. Available from: https://dx.doi.org/10.1100/2011/321979.

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