BÁRTA, Jan, Veronika BÁRTOVÁ, Zbyněk ZDRÁHAL and Ondrej ŠEDO. Cultivar Variability of Patatin Biochemical Characteristics: Table versus Processing Potatoes (Solanum tuberosum L.). Journal of Agricultural and Food Chemistry. WASHINGTON: AMER CHEMICAL SOC, 2012, vol. 60, No 17, p. 4369-4378. ISSN 0021-8561. doi:10.1021/jf3003448.
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Basic information
Original name Cultivar Variability of Patatin Biochemical Characteristics: Table versus Processing Potatoes (Solanum tuberosum L.)
Name in Czech Variabilita odrůd a biochemické charakteristiky patatinů: stolní vs. průmyslové brambory
Authors BÁRTA, Jan (203 Czech Republic, guarantor), Veronika BÁRTOVÁ (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Ondrej ŠEDO (203 Czech Republic, belonging to the institution).
Edition Journal of Agricultural and Food Chemistry, WASHINGTON, AMER CHEMICAL SOC, 2012, 0021-8561.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 40100 4.1 Agriculture, Forestry, and Fisheries
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.906
RIV identification code RIV/00216224:14740/12:00060012
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1021/jf3003448
UT WoS 000303362800026
Keywords in English table and processing potatoes; Solanum tuberosum; cultivar variability; patatin; isoforms; glycosylation; lipid acyl hydrolase activity
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 9. 4. 2013 21:52.
Abstract
Biochemical characteristics of patatin proteins purified by ion-exchange and affinity chromatography from tubers of 20 potato cultivars were studied to evaluate their genotype differences with respect to utility groups, table potato cultivars (TPCs) and processing potato cultivars (PPCs). Both groups of cultivars showed similar values of protein content in dry matter (3.98-7.39%) and of patatin relative abundance (5.40-35.40%). Three mass levels (similar to 40.6, 41.8, and 42.9 kDa) of purified patatins were found by MALDI-TOF MS within all cultivars. Differences among mass levels corresponding with the mass of sugar antenna (similar to 1.2 kDa) confirmed the previous concept of different glycosylation extentsin patatin proteins It was showed that the individual types of patatin varying in their masses occur in the,patatin family in a ratio specific for each of the cultivars, with the lowest mass type being the major one Electrophoretic analyses demonstrated wide cultivar variability in number of patatin forms. Especially 2D-PAGE showed 17-23 detected protein spits independently on the utility group. Specific lipid acyl. hydrolase (LAH) activity of purified patatins from the individual tested cultivars varied between 0.92 and 5.46 mu mol/(min mg). Patatin samples within most of the TPCs exhibited higher values of specific LAH activity than samples of PPCs. It may be supposed that individual patatin forms do not have similar physiological roles.
Abstract (in Czech)
Biochemické charakteristiky patatinů (proteinů z brambor) byly získány po izolaci iontově párovou chromatografií extraktů z hlíz 20 druhů brambor. Izoláty byly srovnávány s ohledem na využití brambor pomocí MALDI-TOF hmotnostní spektrometrie a dvoudimenzionální gelové elektroforézy. Dále byla zkoumána aktivita enzymu lipid acyl. hydrolázy.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
QI91A069, research and development projectName: Hodnocení potenciálu proteinů planých genotypů brambor pro využití ve šlechtění bramboru (Solanum tuberosum L.) a v biotechnologiích.
Investor: Ministry of Agriculture of the CR, Evaluation of wild potato tuber proteins potential for utilization in potato (Solanum tuberosum L.) breeding and biotechnological applications, Sustainable development of agrarian sector
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