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@article{983248, author = {Bárta, Jan and Bártová, Veronika and Zdráhal, Zbyněk and Šedo, Ondrej}, article_location = {WASHINGTON}, article_number = {17}, doi = {http://dx.doi.org/10.1021/jf3003448}, keywords = {table and processing potatoes; Solanum tuberosum; cultivar variability; patatin; isoforms; glycosylation; lipid acyl hydrolase activity}, language = {eng}, issn = {0021-8561}, journal = {Journal of Agricultural and Food Chemistry}, title = {Cultivar Variability of Patatin Biochemical Characteristics: Table versus Processing Potatoes (Solanum tuberosum L.)}, url = {http://pubs.acs.org/doi/abs/10.1021/jf3003448}, volume = {60}, year = {2012} }
TY - JOUR ID - 983248 AU - Bárta, Jan - Bártová, Veronika - Zdráhal, Zbyněk - Šedo, Ondrej PY - 2012 TI - Cultivar Variability of Patatin Biochemical Characteristics: Table versus Processing Potatoes (Solanum tuberosum L.) JF - Journal of Agricultural and Food Chemistry VL - 60 IS - 17 SP - 4369-4378 EP - 4369-4378 PB - AMER CHEMICAL SOC SN - 00218561 KW - table and processing potatoes KW - Solanum tuberosum KW - cultivar variability KW - patatin KW - isoforms KW - glycosylation KW - lipid acyl hydrolase activity UR - http://pubs.acs.org/doi/abs/10.1021/jf3003448 L2 - http://pubs.acs.org/doi/abs/10.1021/jf3003448 N2 - Biochemical characteristics of patatin proteins purified by ion-exchange and affinity chromatography from tubers of 20 potato cultivars were studied to evaluate their genotype differences with respect to utility groups, table potato cultivars (TPCs) and processing potato cultivars (PPCs). Both groups of cultivars showed similar values of protein content in dry matter (3.98-7.39%) and of patatin relative abundance (5.40-35.40%). Three mass levels (similar to 40.6, 41.8, and 42.9 kDa) of purified patatins were found by MALDI-TOF MS within all cultivars. Differences among mass levels corresponding with the mass of sugar antenna (similar to 1.2 kDa) confirmed the previous concept of different glycosylation extentsin patatin proteins It was showed that the individual types of patatin varying in their masses occur in the,patatin family in a ratio specific for each of the cultivars, with the lowest mass type being the major one Electrophoretic analyses demonstrated wide cultivar variability in number of patatin forms. Especially 2D-PAGE showed 17-23 detected protein spits independently on the utility group. Specific lipid acyl. hydrolase (LAH) activity of purified patatins from the individual tested cultivars varied between 0.92 and 5.46 mu mol/(min mg). Patatin samples within most of the TPCs exhibited higher values of specific LAH activity than samples of PPCs. It may be supposed that individual patatin forms do not have similar physiological roles. ER -
BÁRTA, Jan, Veronika BÁRTOVÁ, Zbyněk ZDRÁHAL and Ondrej ŠEDO. Cultivar Variability of Patatin Biochemical Characteristics: Table versus Processing Potatoes (Solanum tuberosum L.). \textit{Journal of Agricultural and Food Chemistry}. WASHINGTON: AMER CHEMICAL SOC, vol.~60, No~17, p.~4369-4378. ISSN~0021-8561. doi:10.1021/jf3003448. 2012.
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