Genomic diversity in two related plant species with and without
sex chromosomes – Silene latifolia and S. vulgaris.

J 2012

Genomic diversity in two related plant species with and without sex chromosomes – Silene latifolia and S. vulgaris.

CEGAN, R, Boris VYSKOT, Eduard KEJNOVSKÝ, Zdeněk KUBÁT, Hana BLAVET et. al.

Basic information

Original name

Genomic diversity in two related plant species with and without sex chromosomes – Silene latifolia and S. vulgaris.

Authors

CEGAN, R, Boris VYSKOT, Eduard KEJNOVSKÝ, Zdeněk KUBÁT, Hana BLAVET, Jan SAFAR, Jaroslav DOLEŽEL, N BLAVET and Roman HOBZA

Edition

PLoS Biology, USA, PUBLIC LIBRARY SCIENCE, 2012, 1544-9173

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 12.472 in 2010

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1371/journal.pone.0031898

UT WoS

000303003500026

Keywords in English

Y-CHROMOSOME; DNA-SEQUENCES; TRANSPOSABLE ELEMENTS; LTR RETROTRANSPOSONS; REPETITIVE DNA; SIZE; EVOLUTION; ACCUMULATION; SYSTEM; GENE

Abstract

V originále

We analyzed differences in the number and distribution of major repetitive DNA elements in two closely related species, Silene latifolia and S. vulgaris. Both species are diploid and possess the same chromosome number (2n = 24), but differ in their genome size and mode of reproduction. The dioecious S. latifolia (1C = 2.70 pg DNA) possesses sex chromosomes and its genome is 2.5x larger than that of the gynodioecious S. vulgaris (1C = 1.13 pg DNA), which does not possess sex chromosomes. We discovered that the genome of S. latifolia is larger mainly due to the expansion of Ogre retrotransposons. Surprisingly, the centromeric STAR-C and TR1 tandem repeats were found to be more abundant in S. vulgaris, the species with the smaller genome. We further examined the distribution of major repetitive sequences in related species in the Caryophyllaceae family. The results of FISH (fluorescence in situ hybridization) on mitotic chromosomes with the Retand element indicate that large rearrangements occurred during the evolution of the Caryophyllaceae family.

Citovat

CEGAN, R, Boris VYSKOT, Eduard KEJNOVSKÝ, Zdeněk KUBÁT, Hana BLAVET, Jan SAFAR, Jaroslav DOLEŽEL, N BLAVET and Roman HOBZA. Genomic diversity in two related plant species with and without sex chromosomes – Silene latifolia and S. vulgaris. PLoS Biology. USA: PUBLIC LIBRARY SCIENCE, 2012, vol. 7, No 2, p. 1-9. ISSN 1544-9173. Available from: https://dx.doi.org/10.1371/journal.pone.0031898.

@article{983308,
   author = {Cegan, R and Vyskot, Boris and Kejnovský, Eduard and Kubát, Zdeněk and Blavet, Hana and Safar, Jan and Doležel, Jaroslav and Blavet, N and Hobza, Roman},
   article_location = {USA},
   article_number = {2},
   doi = {http://dx.doi.org/10.1371/journal.pone.0031898},
   keywords = {Y-CHROMOSOME; DNA-SEQUENCES; TRANSPOSABLE ELEMENTS; LTR RETROTRANSPOSONS; REPETITIVE DNA; SIZE; EVOLUTION; ACCUMULATION; SYSTEM; GENE},
   language = {eng},
   issn = {1544-9173},
   journal = {PLoS Biology},
   title = {Genomic diversity in two related plant species with and without sex chromosomes – Silene latifolia and S. vulgaris.},
   url = {http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.0031898;jsessionid=0D93D2FB30FEFBF4B47D06A24712B1AE},
   volume = {7},
   year = {2012}
}

TY  - JOUR
ID  - 983308
AU  - Cegan, R - Vyskot, Boris - Kejnovský, Eduard - Kubát, Zdeněk - Blavet, Hana - Safar, Jan - Doležel, Jaroslav - Blavet, N - Hobza, Roman
PY  - 2012
TI  - Genomic diversity in two related plant species with and without sex chromosomes – Silene latifolia and S. vulgaris.
JF  - PLoS Biology
VL  - 7
IS  - 2
SP  - 1-9
EP  - 1-9
PB  - PUBLIC LIBRARY SCIENCE
SN  - 15449173
KW  - Y-CHROMOSOME
KW  - DNA-SEQUENCES
KW  - TRANSPOSABLE ELEMENTS
KW  - LTR RETROTRANSPOSONS
KW  - REPETITIVE DNA
KW  - SIZE
KW  - EVOLUTION
KW  - ACCUMULATION
KW  - SYSTEM
KW  - GENE
UR  - http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.0031898;jsessionid=0D93D2FB30FEFBF4B47D06A24712B1AE
N2  - We analyzed differences in the number and distribution of major repetitive DNA elements in two closely related species, Silene latifolia and S. vulgaris. Both species are diploid and possess the same chromosome number (2n = 24), but differ in their genome size and mode of reproduction. The dioecious S. latifolia (1C = 2.70 pg DNA) possesses sex chromosomes and its genome is 2.5x larger than that of the gynodioecious S. vulgaris (1C = 1.13 pg DNA), which does not possess sex chromosomes. We discovered that the genome of S. latifolia is larger mainly due to the expansion of Ogre retrotransposons. Surprisingly, the centromeric STAR-C and TR1 tandem repeats were found to be more abundant in S. vulgaris, the species with the smaller genome. We further examined the distribution of major repetitive sequences in related species in the Caryophyllaceae family. The results of FISH (fluorescence in situ hybridization) on mitotic chromosomes with the Retand element indicate that large rearrangements occurred during the evolution of the Caryophyllaceae family.
ER  -

CEGAN, R, Boris VYSKOT, Eduard KEJNOVSKÝ, Zdeněk KUBÁT, Hana BLAVET, Jan SAFAR, Jaroslav DOLEŽEL, N BLAVET and Roman HOBZA. Genomic diversity in two related plant species with and without sex chromosomes – Silene latifolia and S. vulgaris. \textit{PLoS Biology}. USA: PUBLIC LIBRARY SCIENCE, 2012, vol.~7, No~2, p.~1-9. ISSN~1544-9173. Available from: https://dx.doi.org/10.1371/journal.pone.0031898.

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