Arrangement of nuclear structures is not transmitted through
mitosis but is identical in sister cells

J 2012

Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells

ORLOVA, Darya Yurevna, Lenka STIXOVÁ, Stanislav KOZUBEK, Hinco GIERMAN, Gabriela ŠUSTÁČKOVÁ et. al.

Basic information

Original name

Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells

Authors

ORLOVA, Darya Yurevna (643 Russian Federation), Lenka STIXOVÁ (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Hinco GIERMAN (840 United States of America), Gabriela ŠUSTÁČKOVÁ (203 Czech Republic), Andrei CHERNYSHEV (643 Russian Federation), Ruslan MEDVEDEV (643 Russian Federation), Soňa LEGARTOVÁ (703 Slovakia), Rogier VERSTEEG (528 Netherlands), Pavel MATULA (203 Czech Republic, guarantor, belonging to the institution), Roman STOKLASA (703 Slovakia, belonging to the institution) and Eva BÁRTOVÁ (203 Czech Republic)

Edition

Journal of Cellular Biochemistry, 2012, 0730-2312

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.062

RIV identification code

RIV/00216224:14330/12:00057413

Organization unit

Faculty of Informatics

DOI

http://dx.doi.org/10.1002/jcb.24208

UT WoS

000308927200002

Keywords (in Czech)

histones; chromatin; HP1 protein; photoconversion; Dendra2; Cajal bodies

Keywords in English

histones; chromatin; HP1 protein; photoconversion; Dendra2; Cajal bodies

Abstract

V originále

Although it is well known that chromosomes are non-randomly organized during interphase, it is not completely clear whether higher-order chromatin structure is transmitted from mother to daughter cells. Therefore, we addressed the question of how chromatin is rearranged during interphase and whether heterochromatin pattern is transmitted after mitosis. We additionally tested the similarity of chromatin arrangement in sister interphase nuclei. We noticed a very active cell rotation during interphase, especially when histone hyperacetylation was induced or transcription was inhibited. This natural phenomenon can influence the analysis of nuclear arrangement. Using photoconversion of Dendra2-tagged core histone H4 we showed that the distribution of chromatin in daughter interphase nuclei differed from that in mother cells. Similarly, the nuclear distribution of heterochromatin protein 1beta (HP1beta) was not completely identical in mother and daughter cells. However, identity between mother and daughter cells was in many cases evidenced by nucleolar composition. Moreover, morphology of nucleoli, HP1beta protein, Cajal bodies, chromosome territories, and gene transcripts were identical in sister cell nuclei. We conclude that the arrangement of interphase chromatin is not transmitted through mitosis, but the chromatin pattern is identical in naturally synchronized sister cells. It is also necessary to take into account the possibility that cell rotation and the degree of chromatin condensation during functionally specific cell cycle phases might influence our view of nuclear architecture.

Links

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

MUNI/A/0914/2009, interní kód MU

Name: Rozsáhlé výpočetní systémy: modely, aplikace a verifikace (Acronym: SV-FI MAV)

Investor: Masaryk University, Category A

Citovat

ORLOVA, Darya Yurevna, Lenka STIXOVÁ, Stanislav KOZUBEK, Hinco GIERMAN, Gabriela ŠUSTÁČKOVÁ, Andrei CHERNYSHEV, Ruslan MEDVEDEV, Soňa LEGARTOVÁ, Rogier VERSTEEG, Pavel MATULA, Roman STOKLASA and Eva BÁRTOVÁ. Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells. Journal of Cellular Biochemistry. 2012, vol. 113, No 11, p. 3313-3329. ISSN 0730-2312. Available from: https://dx.doi.org/10.1002/jcb.24208.

@article{983372,
   author = {Orlova, Darya Yurevna and Stixová, Lenka and Kozubek, Stanislav and Gierman, Hinco and Šustáčková, Gabriela and Chernyshev, Andrei and Medvedev, Ruslan and Legartová, Soňa and Versteeg, Rogier and Matula, Pavel and Stoklasa, Roman and Bártová, Eva},
   article_number = {11},
   doi = {http://dx.doi.org/10.1002/jcb.24208},
   keywords = {histones; chromatin; HP1 protein; photoconversion; Dendra2; Cajal bodies},
   language = {eng},
   issn = {0730-2312},
   journal = {Journal of Cellular Biochemistry},
   title = {Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract},
   volume = {113},
   year = {2012}
}

TY  - JOUR
ID  - 983372
AU  - Orlova, Darya Yurevna - Stixová, Lenka - Kozubek, Stanislav - Gierman, Hinco - Šustáčková, Gabriela - Chernyshev, Andrei - Medvedev, Ruslan - Legartová, Soňa - Versteeg, Rogier - Matula, Pavel - Stoklasa, Roman - Bártová, Eva
PY  - 2012
TI  - Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells
JF  - Journal of Cellular Biochemistry
VL  - 113
IS  - 11
SP  - 3313-3329
EP  - 3313-3329
SN  - 07302312
KW  - histones
KW  - chromatin
KW  - HP1 protein
KW  - photoconversion
KW  - Dendra2
KW  - Cajal bodies
UR  - http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract
N2  - Although it is well known that chromosomes are non-randomly organized during interphase, it is not completely clear whether higher-order chromatin structure is transmitted from mother to daughter cells. Therefore, we addressed the question of how chromatin is rearranged during interphase and whether heterochromatin pattern is transmitted after mitosis. We additionally tested the similarity of chromatin arrangement in sister interphase nuclei. We noticed a very active cell rotation during interphase, especially when histone hyperacetylation was induced or transcription was inhibited. This natural phenomenon can influence the analysis of nuclear arrangement. Using photoconversion of Dendra2-tagged core histone H4 we showed that the distribution of chromatin in daughter interphase nuclei differed from that in mother cells. Similarly, the nuclear distribution of heterochromatin protein 1beta (HP1beta) was not completely identical in mother and daughter cells. However, identity between mother and daughter cells was in many cases evidenced by nucleolar composition. Moreover, morphology of nucleoli, HP1beta protein, Cajal bodies, chromosome territories, and gene transcripts were identical in sister cell nuclei. We conclude that the arrangement of interphase chromatin is not transmitted through mitosis, but the chromatin pattern is identical in naturally synchronized sister cells. It is also necessary to take into account the possibility that cell rotation and the degree of chromatin condensation during functionally specific cell cycle phases might influence our view of nuclear architecture.
ER  -

ORLOVA, Darya Yurevna, Lenka STIXOVÁ, Stanislav KOZUBEK, Hinco GIERMAN, Gabriela ŠUSTÁČKOVÁ, Andrei CHERNYSHEV, Ruslan MEDVEDEV, Soňa LEGARTOVÁ, Rogier VERSTEEG, Pavel MATULA, Roman STOKLASA and Eva BÁRTOVÁ. Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells. \textit{Journal of Cellular Biochemistry}. 2012, vol.~113, No~11, p.~3313-3329. ISSN~0730-2312. Available from: https://dx.doi.org/10.1002/jcb.24208.

Detailed Information on Publication Record