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@article{983372,
author = {Orlova, Darya Yurevna and Stixová, Lenka and Kozubek, Stanislav and Gierman, Hinco and Šustáčková, Gabriela and Chernyshev, Andrei and Medvedev, Ruslan and Legartová, Soňa and Versteeg, Rogier and Matula, Pavel and Stoklasa, Roman and Bártová, Eva},
article_number = {11},
doi = {http://dx.doi.org/10.1002/jcb.24208},
keywords = {histones; chromatin; HP1 protein; photoconversion; Dendra2; Cajal bodies},
language = {eng},
issn = {0730-2312},
journal = {Journal of Cellular Biochemistry},
title = {Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells},
url = {http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract},
volume = {113},
year = {2012}
}
TY - JOUR
ID - 983372
AU - Orlova, Darya Yurevna - Stixová, Lenka - Kozubek, Stanislav - Gierman, Hinco - Šustáčková, Gabriela - Chernyshev, Andrei - Medvedev, Ruslan - Legartová, Soňa - Versteeg, Rogier - Matula, Pavel - Stoklasa, Roman - Bártová, Eva
PY - 2012
TI - Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells
JF - Journal of Cellular Biochemistry
VL - 113
IS - 11
SP - 3313-3329
EP - 3313-3329
SN - 07302312
KW - histones
KW - chromatin
KW - HP1 protein
KW - photoconversion
KW - Dendra2
KW - Cajal bodies
UR - http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract
L2 - http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract
N2 - Although it is well known that chromosomes are non-randomly organized during interphase, it is not completely clear whether higher-order chromatin structure is transmitted from mother to daughter cells. Therefore, we addressed the question of how chromatin is rearranged during interphase and whether heterochromatin pattern is transmitted after mitosis. We additionally tested the similarity of chromatin arrangement in sister interphase nuclei. We noticed a very active cell rotation during interphase, especially when histone hyperacetylation was induced or transcription was inhibited. This natural phenomenon can influence the analysis of nuclear arrangement. Using photoconversion of Dendra2-tagged core histone H4 we showed that the distribution of chromatin in daughter interphase nuclei differed from that in mother cells. Similarly, the nuclear distribution of heterochromatin protein 1beta (HP1beta) was not completely identical in mother and daughter cells. However, identity between mother and daughter cells was in many cases evidenced by nucleolar composition. Moreover, morphology of nucleoli, HP1beta protein, Cajal bodies, chromosome territories, and gene transcripts were identical in sister cell nuclei. We conclude that the arrangement of interphase chromatin is not transmitted through mitosis, but the chromatin pattern is identical in naturally synchronized sister cells. It is also necessary to take into account the possibility that cell rotation and the degree of chromatin condensation during functionally specific cell cycle phases might influence our view of nuclear architecture.
ER -
ORLOVA, Darya Yurevna, Lenka STIXOVÁ, Stanislav KOZUBEK, Hinco GIERMAN, Gabriela ŠUSTÁČKOVÁ, Andrei CHERNYSHEV, Ruslan MEDVEDEV, Soňa LEGARTOVÁ, Rogier VERSTEEG, Pavel MATULA, Roman STOKLASA and Eva BÁRTOVÁ. Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells. \textit{Journal of Cellular Biochemistry}. 2012, vol.~113, No~11, p.~3313-3329. ISSN~0730-2312. Available from: https://dx.doi.org/10.1002/jcb.24208.
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