CASTILLO, Gabriela, Libuše TRNKOVÁ and Tibor HIANIK. GOLD CD-SENSOR PLATFORM FOR DNA APTAMER ASSEMBLING TOWARDS HUMAN THROMBIN RECOGNITION. In XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Brno: Mendelova univerzita v Brně, 2012, p. 28-29. ISBN 978-80-7375-618-5.
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Basic information
Original name GOLD CD-SENSOR PLATFORM FOR DNA APTAMER ASSEMBLING TOWARDS HUMAN THROMBIN RECOGNITION
Authors CASTILLO, Gabriela (218 Ecuador), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution) and Tibor HIANIK (703 Slovakia, guarantor).
Edition Brno, XII. Pracovní setkání fyzikálních chemiků a elektrochemiků, p. 28-29, 2 pp. 2012.
Publisher Mendelova univerzita v Brně
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10403 Physical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
RIV identification code RIV/00216224:14310/12:00060052
Organization unit Faculty of Science
ISBN 978-80-7375-618-5
Keywords in English thrombin;DNA;gold electrode;BSA;SEM;biosensor
Tags AKR, rivok
Changed by Changed by: Mgr. Sylvie Dohnalíková, učo 150911. Changed: 17/1/2013 09:12.
Abstract
In this work, we report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance spectroscopy (EIS) upon aptamer-protein formation at the surface of gold CDtrodes (GCDTs) in the presence of redox couple [Fe(CN)6]3-/4- [2]. The biosensor was constructed by self-assembling a thiol-modified thrombin binding aptamer (TBA) onto the GCDT surface followed by the protein addition in a wide range of concentrations ranging from 20 nM to 1 microM. Achieved results reveal that the GCDTs present good versatility and suitable reproducibility for thrombin recognition with a limit of detection (LOD) of 5 nM. Non-specific interaction with similar molecular compounds like bovine serum albumin (BSA) demonstrates an appropriate and specifically target sensor response thanks to the aptamer incorporation as transducer elements. In order to verify the practical application of GCDT aptasensor in complex biological fluids, we have detected thrombin in spiked diluted blood plasma samples. Additionally, individual steps of biosensor assembling were monitored by Scanning electron microscopy (SEM). GCDT response was also satisfactory compared with commercial gold screen printed electrodes (GSPE).
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