Other formats:
BibTeX
LaTeX
RIS
@inproceedings{983975, author = {Castillo, Gabriela and Trnková, Libuše and Hianik, Tibor}, address = {Brno}, booktitle = {XII. Pracovní setkání fyzikálních chemiků a elektrochemiků}, keywords = {thrombin;DNA;gold electrode;BSA;SEM;biosensor}, howpublished = {tištěná verze "print"}, language = {eng}, location = {Brno}, isbn = {978-80-7375-618-5}, pages = {28-29}, publisher = {Mendelova univerzita v Brně}, title = {GOLD CD-SENSOR PLATFORM FOR DNA APTAMER ASSEMBLING TOWARDS HUMAN THROMBIN RECOGNITION}, year = {2012} }
TY - JOUR ID - 983975 AU - Castillo, Gabriela - Trnková, Libuše - Hianik, Tibor PY - 2012 TI - GOLD CD-SENSOR PLATFORM FOR DNA APTAMER ASSEMBLING TOWARDS HUMAN THROMBIN RECOGNITION PB - Mendelova univerzita v Brně CY - Brno SN - 9788073756185 KW - thrombin;DNA;gold electrode;BSA;SEM;biosensor N2 - In this work, we report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance spectroscopy (EIS) upon aptamer-protein formation at the surface of gold CDtrodes (GCDTs) in the presence of redox couple [Fe(CN)6]3-/4- [2]. The biosensor was constructed by self-assembling a thiol-modified thrombin binding aptamer (TBA) onto the GCDT surface followed by the protein addition in a wide range of concentrations ranging from 20 nM to 1 microM. Achieved results reveal that the GCDTs present good versatility and suitable reproducibility for thrombin recognition with a limit of detection (LOD) of 5 nM. Non-specific interaction with similar molecular compounds like bovine serum albumin (BSA) demonstrates an appropriate and specifically target sensor response thanks to the aptamer incorporation as transducer elements. In order to verify the practical application of GCDT aptasensor in complex biological fluids, we have detected thrombin in spiked diluted blood plasma samples. Additionally, individual steps of biosensor assembling were monitored by Scanning electron microscopy (SEM). GCDT response was also satisfactory compared with commercial gold screen printed electrodes (GSPE). ER -
CASTILLO, Gabriela, Libuše TRNKOVÁ and Tibor HIANIK. GOLD CD-SENSOR PLATFORM FOR DNA APTAMER ASSEMBLING TOWARDS HUMAN THROMBIN RECOGNITION. In \textit{XII. Pracovní setkání fyzikálních chemiků a elektrochemiků}. Brno: Mendelova univerzita v Brně, 2012, p.~28-29. ISBN~978-80-7375-618-5.
|