Detection of melamine in infant formula and grain powder by surface-assisted laser desorption/ionization mass spectrometry

HSIEH, Yi-Ting, Wen-Tsen CHEN, Iva TOMALOVÁ, Jan PREISLER and Huan-Tsung CHANG. Detection of melamine in infant formula and grain powder by surface-assisted laser desorption/ionization mass spectrometry. Rapid Communications in Mass Spectrometry. Wiley InterScience, 2012, vol. 26, No 12, p. 1393-1398. ISSN 0951-4198. Available from: https://dx.doi.org/10.1002/rcm.6238.

Basic information

• Original name: Detection of melamine in infant formula and grain powder by surface-assisted laser desorption/ionization mass spectrometry
• Authors: HSIEH, Yi-Ting (158 Taiwan), Wen-Tsen CHEN (158 Taiwan), Iva TOMALOVÁ (203 Czech Republic, belonging to the institution), Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution) and Huan-Tsung CHANG (158 Taiwan).
• Edition: Rapid Communications in Mass Spectrometry, Wiley InterScience, 2012, 0951-4198.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.509
• RIV identification code: RIV/00216224:14740/12:00057422
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/rcm.6238
• UT WoS: 000304154300001
• Keywords in English: Melamine; Gold Nanoparticles; SALDI MS
• Tags: ok, rivok
• Tags: Reviewed

Abstract

We have developed a method for the determination of melamine (MEL), ammeline (AMN), and ammelide (AMD) by surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) using gold nanoparticles (Au NPs). The major peaks for MEL, AMN, and AMD at m/z 127.07, 128.05, and 129.04 are assigned to the [MEL + H]+, [AMN + H]+, and [AMD + H]+ ions. Because the three tested compounds adsorb weakly onto the surfaces of the Au NPs through Au–N bonding, they can be easily concentrated from complex samples by applying a simple trapping/centrifugation process. The SALDI-MS method provides limits of detection of 5, 10, and 300 nM for MEL, AMN, and AMD, respectively, at a signal-to-noise ratio of 3. The signal variation for 150-shot average spectra of the three analytes within the same spot was 15%, and the batch-to-batch variation was 20%. We have validated the practicality of this approach by the analysis of these three analytes in infant formula and grain powder. This simple and rapid SALDI-MS approach holds great potential for screening of MEL in foods.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• GCP206/10/J012, research and development project: Name: Analýza biomolekul hmotnostní spektrometrií s laserovou desorpcí/ionizací za účasti nanomateriálů

Investor: Czech Science Foundation