PILAŘOVÁ, Iveta, Gabriela CASTILLO and Libuše TRNKOVÁ. SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES. In XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků. Brno: Mendelova univerzita v Brně, 2012, p. 216-219. ISBN 978-80-7375-618-5.
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Basic information
Original name SPECTROSCOPIC CHARACTERIZATION OF GOLD NANOPARTICLES
Authors PILAŘOVÁ, Iveta (203 Czech Republic, belonging to the institution), Gabriela CASTILLO (218 Ecuador) and Libuše TRNKOVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition Brno, XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků. p. 216-219, 4 pp. 2012.
Publisher Mendelova univerzita v Brně
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10403 Physical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
RIV identification code RIV/00216224:14740/12:00057434
Organization unit Central European Institute of Technology
ISBN 978-80-7375-618-5
Keywords in English Gold nanoparticles;GNPs;UV-Vis spectroscopy;PCS;thrombin;aptamer
Tags rivok
Changed by Changed by: Mgr. Nikola Kostlánová, Ph.D., učo 12689. Changed: 12/4/2013 14:50.
Abstract
Gold nanoparticles (GNPs) are the object of interest in the field of nanotechnology due to their unique electronic, structural, optical, magnetic, thermal, and catalytic properties. They are extensively applied in the construction of electrochemical sensors and biosensors. The aim of this contribution is to characterize GNPs of both sizes (diameters of 5nm and 10 nm) in buffered and non-buffered aqueous solutions with the change of ionic strength by spectroscopic methods (UV/ Vis, photon correlation spectroscopy), and to investigate the GNP–aptamer–thrombin system as an application of GNPs in a sensitive aptasensor. For the immobilization the thrombin-binding aptamer (TBA) with the sequence GGT TGG TGT GGT TGG on the GNP surface was modified by a sulfhydryl group. The study showed that the spectral characterization of GNPs depends strongly not only on the aptamer–thrombin conjugate but also on the GNP size and solution composition (buffer, ions and their concentrations).
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GD106/09/H035, research and development projectName: Víceúrovňový design pokrokových materiálů
Investor: Czech Science Foundation
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