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@inproceedings{984226,
author = {Šobrová, Pavlína and Ryvolová, Markéta and Hynek, David and Adam, Vojtěch and Hubálek, Jaromír and Trnková, Libuše and Kizek, René},
address = {Brno},
booktitle = {XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků.},
keywords = {prion protein;cyclic voltammetry;differential pulse voltammetry;Brdicka reaction;chronopotentiometry},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Brno},
isbn = {978-80-7375-618-5},
pages = {234-236},
publisher = {Mendelova univerzita v Brně},
title = {INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE},
year = {2012}
}
TY - JOUR
ID - 984226
AU - Šobrová, Pavlína - Ryvolová, Markéta - Hynek, David - Adam, Vojtěch - Hubálek, Jaromír - Trnková, Libuše - Kizek, René
PY - 2012
TI - INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE
PB - Mendelova univerzita v Brně
CY - Brno
SN - 9788073756185
KW - prion protein;cyclic voltammetry;differential pulse voltammetry;Brdicka reaction;chronopotentiometry
N2 - Electrochemical study of Beta-sheet breaker prion protein is the main aim of this study. For this purpose, cyclic voltammetry (CV), differential pulse voltammetry, differential pulse voltammetry Brdicka reaction and chronopotentiometric stripping analysis were used. Under the optimal conditions (phosphate buffer, pH 7.38 and time of accumulation 100 s), detected by DPV prion was characterized using different techniques and their limits of detection were found. Adsorptive transfer stripping technique coupled with the abovementioned methods offers very lower detection limits. The lowest limits of detection were determined by CPSA AdTS i.e. 25 pmol in volume of 5 microl. CPSA is therefore a very sensitive tool for the studying of prion behaviour. Moreover, the influence of heat denaturation was observed. It clearly follows from the results obtained that signals of prion decreased linearly depending on the duration of the heat treatment at 99 C for various time intervals: 0, 15, 30, 45, and 60 min. The correlation coefficients of the measured dependencies as 0.9929, 0.9973, 0.9965 and 0.9957 were determined by CV, DPV, DPV Brdicka reaction and the most sensitive CPSA, respectively.
ER -
ŠOBROVÁ, Pavlína, Markéta RYVOLOVÁ, David HYNEK, Vojtěch ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ and René KIZEK. INFLUENCE OF HEAT DENATURATION OF Beta-SHEET BREAKER PRION PROTEIN ON ELECTROCHEMICAL RESPONCE. In \textit{XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků.}. Brno: Mendelova univerzita v Brně, 2012, p.~234-236. ISBN~978-80-7375-618-5.
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