ŠVEC, Pavel and Hana BRYNDOVÁ. Enterococcus spp. associated with plants. In 31st Annual Meeting of the European Culture Collections' Organization (ECCO XXXI). 2012. ISBN 978-972-97916-5-9.
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Basic information
Original name Enterococcus spp. associated with plants
Authors ŠVEC, Pavel (203 Czech Republic, guarantor, belonging to the institution) and Hana BRYNDOVÁ (203 Czech Republic, belonging to the institution).
Edition 31st Annual Meeting of the European Culture Collections' Organization (ECCO XXXI), 2012.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/12:00060295
Organization unit Faculty of Science
ISBN 978-972-97916-5-9
Keywords in English Enterococcus; plants; taxonomy; identification
Changed by Changed by: doc. RNDr. Pavel Švec, Ph.D., učo 1098. Changed: 28/6/2012 07:39.
Abstract
A group of 104 presumptive enterococcal strains was isolated on Kanamycin esculin azide agar in the frame of a study dealing with investigation of Enterococcus spp. populations occurring on plants. Isolated strains were analysed using rep-PCR with the (GTG)5 primer and obtained fingerprints were compared with an in-house CCM reference database containing more that 5000 fingerprints representing multiple Gram-positive bacterial species including all known Enterococcus spp. In total, 53 strains matched reference database entries and were identified as Enterococcus faecalis (18 strains), Enterococcus haemoperoxidus (13), Enterococcus casseliflavus (4), Enterococcus faecium (2), Enterococcus moraviensis (2), Enterococcus mundtii (2), and Enterococcus durans (1). Two groups of strains were assigned as recently proposed species Enterococcus ureilyticus (7) and Enterococcus rotai (4). Another group of initially unidentified eight plant associated strains was characterized in detail and described recently as Enterococcus plantarum. Remaining nine strains were identified as members of other genera (Lactococcus, Lactobacillus, Leuconostoc) or were not clustered with any fingerprints included in the database (34 strains). Overall results obtained in the frame of the present study revealed that plants are inhabited by diverse Enterococcus spp. A high proportion of unidentified strains and isolation of representatives of recently proposed Enterococcus spp. imply that unknown species occur within the taxonomically rich enterococcal populations associated with plants.
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MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
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