| CASTILLO, Gabriela, Libuše TRNKOVÁ, Radim HRDÝ a Tibor HIANIK. Impedimetric Aptasensor for Thrombin Recognition Based on CD Support. Electroanalysis. 2012, roč. 24, č. 5, s. 1079-1087. ISSN 1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201200041. |
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@article{986053,
author = {Castillo, Gabriela and Trnková, Libuše and Hrdý, Radim and Hianik, Tibor},
article_number = {5},
doi = {http://dx.doi.org/10.1002/elan.201200041},
keywords = {DNA aptamers; Biosensors; Human thrombin; Electrochemical impedance spectroscopy; Gold CD-trodes},
language = {eng},
issn = {1040-0397},
journal = {Electroanalysis},
title = {Impedimetric Aptasensor for Thrombin Recognition Based on CD Support},
volume = {24},
year = {2012}
}
TY - JOUR
ID - 986053
AU - Castillo, Gabriela - Trnková, Libuše - Hrdý, Radim - Hianik, Tibor
PY - 2012
TI - Impedimetric Aptasensor for Thrombin Recognition Based on CD Support
JF - Electroanalysis
VL - 24
IS - 5
SP - 1079-1087
EP - 1079-1087
SN - 10400397
KW - DNA aptamers
KW - Biosensors
KW - Human thrombin
KW - Electrochemical impedance spectroscopy
KW - Gold CD-trodes
N2 - We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein formation at the surface of CD-trodes (GCDTs) in the presence of the redox couple [Fe(CN)6]3-/4-. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self-assembling of a thiol-modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 mu M with a limit of detection of 5 nM.
ER -
CASTILLO, Gabriela, Libuše TRNKOVÁ, Radim HRDÝ a Tibor HIANIK. Impedimetric Aptasensor for Thrombin Recognition Based on CD Support. \textit{Electroanalysis}. 2012, roč.~24, č.~5, s.~1079-1087. ISSN~1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201200041.
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