CASTILLO, Gabriela, Libuše TRNKOVÁ, Radim HRDÝ a Tibor HIANIK. Impedimetric Aptasensor for Thrombin Recognition Based on CD Support. Electroanalysis. 2012, roč. 24, č. 5, s. 1079-1087. ISSN 1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201200041. |
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@article{986053, author = {Castillo, Gabriela and Trnková, Libuše and Hrdý, Radim and Hianik, Tibor}, article_number = {5}, doi = {http://dx.doi.org/10.1002/elan.201200041}, keywords = {DNA aptamers; Biosensors; Human thrombin; Electrochemical impedance spectroscopy; Gold CD-trodes}, language = {eng}, issn = {1040-0397}, journal = {Electroanalysis}, title = {Impedimetric Aptasensor for Thrombin Recognition Based on CD Support}, volume = {24}, year = {2012} }
TY - JOUR ID - 986053 AU - Castillo, Gabriela - Trnková, Libuše - Hrdý, Radim - Hianik, Tibor PY - 2012 TI - Impedimetric Aptasensor for Thrombin Recognition Based on CD Support JF - Electroanalysis VL - 24 IS - 5 SP - 1079-1087 EP - 1079-1087 SN - 10400397 KW - DNA aptamers KW - Biosensors KW - Human thrombin KW - Electrochemical impedance spectroscopy KW - Gold CD-trodes N2 - We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein formation at the surface of CD-trodes (GCDTs) in the presence of the redox couple [Fe(CN)6]3-/4-. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self-assembling of a thiol-modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 mu M with a limit of detection of 5 nM. ER -
CASTILLO, Gabriela, Libuše TRNKOVÁ, Radim HRDÝ a Tibor HIANIK. Impedimetric Aptasensor for Thrombin Recognition Based on CD Support. \textit{Electroanalysis}. 2012, roč.~24, č.~5, s.~1079-1087. ISSN~1040-0397. Dostupné z: https://dx.doi.org/10.1002/elan.201200041.
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