CASTILLO, Gabriela, Libuše TRNKOVÁ, Radim HRDÝ and Tibor HIANIK. Impedimetric Aptasensor for Thrombin Recognition Based on CD Support. Electroanalysis. 2012, vol. 24, No 5, p. 1079-1087. ISSN 1040-0397. Available from: https://dx.doi.org/10.1002/elan.201200041.
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Basic information
Original name Impedimetric Aptasensor for Thrombin Recognition Based on CD Support
Authors CASTILLO, Gabriela (218 Ecuador), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution), Radim HRDÝ (203 Czech Republic) and Tibor HIANIK (703 Slovakia, guarantor).
Edition Electroanalysis, 2012, 1040-0397.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10403 Physical chemistry
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.817
RIV identification code RIV/00216224:14310/12:00060299
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1002/elan.201200041
UT WoS 000303439500013
Keywords in English DNA aptamers; Biosensors; Human thrombin; Electrochemical impedance spectroscopy; Gold CD-trodes
Tags AKR, rivok
Changed by Changed by: Mgr. Sylvie Dohnalíková, učo 150911. Changed: 9/1/2013 10:36.
Abstract
We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein formation at the surface of CD-trodes (GCDTs) in the presence of the redox couple [Fe(CN)6]3-/4-. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self-assembling of a thiol-modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 mu M with a limit of detection of 5 nM.
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