| HYNEK, David, Ludmila KREJČOVÁ, Ondřej ZÍTKA, Vojtěch ADAM, Libuše TRNKOVÁ, Jiří SOCHOR, Marie STIBOROVÁ, Tomáš ECKSCHLAGER, Jaromír HUBÁLEK and René KIZEK. Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I. International Journal of Electrochemical Science. 2012, vol. 7, No 1, p. 13-33. ISSN 1452-3981. |
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@article{986083,
author = {Hynek, David and Krejčová, Ludmila and Zítka, Ondřej and Adam, Vojtěch and Trnková, Libuše and Sochor, Jiří and Stiborová, Marie and Eckschlager, Tomáš and Hubálek, Jaromír and Kizek, René},
article_number = {1},
keywords = {intercalating drug; doxorubicin; hetero-nucleotides; square wave voltammetry; adsorptive transfer stripping technique; DNA},
language = {eng},
issn = {1452-3981},
journal = {International Journal of Electrochemical Science},
title = {Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I},
volume = {7},
year = {2012}
}
TY - JOUR
ID - 986083
AU - Hynek, David - Krejčová, Ludmila - Zítka, Ondřej - Adam, Vojtěch - Trnková, Libuše - Sochor, Jiří - Stiborová, Marie - Eckschlager, Tomáš - Hubálek, Jaromír - Kizek, René
PY - 2012
TI - Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I
JF - International Journal of Electrochemical Science
VL - 7
IS - 1
SP - 13-33
EP - 13-33
SN - 14523981
KW - intercalating drug
KW - doxorubicin
KW - hetero-nucleotides
KW - square wave voltammetry
KW - adsorptive transfer stripping technique
KW - DNA
N2 - Doxorubicin is a known anticancer drug intercalating into dsDNA. The mechanism of interaction of doxorubicin with ssDNA is not clear. In this study, adsorptive transfer stripping technique coupled with square wave voltammetry was used for studying of interaction of doxorubicin with single stranded oligonucleotides (ssODN) in various ratios (1:1, 1:2 and 1:5) and interaction times (0-300 min.). When comparing the results for three ratios of the mixture between drug and ssODN we found that the interval of peak intensities of oligonucleotide reduced with the increasing ratio of oligonucleotide-doxorubicin, i.e. for ratio 1:1 it is 25-85 %, for ratio 1:2 it is 16-60 % and for ratio 1:5 it is 19-51 %. The interval of relative signal intensities of doxorubicin ranges from 8 to 100 % for ratio 1:1, 22-100 % for ratios 1:2 and 1:5. The results showed the assumption that DOXO peak height increased with increasing concentration of the drug intercalated into ssODN, but the signals CA peaks changes during the interaction suggest the changes in the structure of ssODN.
ER -
HYNEK, David, Ludmila KREJČOVÁ, Ondřej ZÍTKA, Vojtěch ADAM, Libuše TRNKOVÁ, Jiří SOCHOR, Marie STIBOROVÁ, Tomáš ECKSCHLAGER, Jaromír HUBÁLEK and René KIZEK. Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I. \textit{International Journal of Electrochemical Science}. 2012, vol.~7, No~1, p.~13-33. ISSN~1452-3981.
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