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@article{987838,
author = {Kohoutek, Jiří and Li, Qintong and Blažek, Dalibor and Luo, Zeping and Jiang, Huimin and Peterlin, B Matija},
article_location = {Washington, D.C.},
article_number = {12},
doi = {http://dx.doi.org/10.1128/MCB.00172-09},
keywords = {RNA-POLYMERASE-II; ELONGATION-FACTOR-B; RECRUITS P-TEFB; TRANSCRIPTIONAL ELONGATION; TERMINAL DOMAIN; TARGET GENES; EXPRESSION; ACTIVATION; TAT; DIFFERENTIATION},
language = {eng},
issn = {0270-7306},
journal = {Molecular and Cellular Biology},
title = {Cyclin T2 Is Essential for Mouse Embryogenesis},
url = {http://mcb.asm.org/content/29/12/3280.full.pdf},
volume = {29},
year = {2009}
}
TY - JOUR
ID - 987838
AU - Kohoutek, Jiří - Li, Qintong - Blažek, Dalibor - Luo, Zeping - Jiang, Huimin - Peterlin, B Matija
PY - 2009
TI - Cyclin T2 Is Essential for Mouse Embryogenesis
JF - Molecular and Cellular Biology
VL - 29
IS - 12
SP - 3280-3285
EP - 3280-3285
PB - ASM
SN - 02707306
KW - RNA-POLYMERASE-II
KW - ELONGATION-FACTOR-B
KW - RECRUITS P-TEFB
KW - TRANSCRIPTIONAL ELONGATION
KW - TERMINAL DOMAIN
KW - TARGET GENES
KW - EXPRESSION
KW - ACTIVATION
KW - TAT
KW - DIFFERENTIATION
UR - http://mcb.asm.org/content/29/12/3280.full.pdf
N2 - The positive transcription elongation factor b (P-TEFb) is essential for the elongation of transcription and cotranscriptional processing by RNA polymerase II. In mammals, it contains predominantly the C-type cyclin cyclin T1 (CycT1) or CycT2 and cyclin-dependent kinase 9 (Cdk9). To determine if these cyclins have redundant functions or affect distinct sets of genes, we genetically inactivated the CycT2 gene (Ccnt2) using the beta-galactosidase-neomycin gene (beta-geo) gene trap technology in the mouse. Visualizing beta-galactosidase during mouse embryogenesis revealed that CycT2 is expressed abundantly during embryogenesis and throughout the organism in the adult. This finding was reflected in the expression of CycT2 in all adult tissues and organs. However, despite numerous matings of heterozygous mice, we observed no CycT2(-/-) embryos, pups, or adult mice. This early lethality could have resulted from decreased expression of critical genes, which were revealed by short interfering RNAs against CycT2 in embryonic stem cells. Thus, CycT1 and CycT2 are not redundant, and these different P-TEFb complexes regulate subsets of distinct genes that are important for embryonic development.
ER -
KOHOUTEK, Jiří, Qintong LI, Dalibor BLAŽEK, Zeping LUO, Huimin JIANG a B Matija PETERLIN. Cyclin T2 Is Essential for Mouse Embryogenesis. \textit{Molecular and Cellular Biology}. Washington, D.C.: ASM, roč.~29, č.~12, s.~3280-3285. ISSN~0270-7306. doi:10.1128/MCB.00172-09. 2009.
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