KOHOUTEK, Jiří, Dalibor BLAŽEK a B Matija PETERLIN. Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: NATL ACAD SCIENCES, roč. 103, č. 46, s. 17349-17354. ISSN 0027-8424. doi:10.1073/pnas.0603079103. 2006.
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Základní údaje
Originální název Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters
Autoři KOHOUTEK, Jiří, Dalibor BLAŽEK a B Matija PETERLIN.
Vydání Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, NATL ACAD SCIENCES, 2006, 0027-8424.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10600 1.6 Biological sciences
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 9.643
Doi http://dx.doi.org/10.1073/pnas.0603079103
UT WoS 000242249400052
Klíčová slova anglicky RNA-POLYMERASE-II; MELANOMA CELL-LINES; P-TEFB; 7SK SNRNA; EXPRESSION; CIITA; COMPLEX; GENES; DIFFERENTIATION; ACTIVATION
Štítky ok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Olga Křížová, učo 56639. Změněno: 24. 7. 2012 05:54.
Anotace
The class II transactivator (CIITA) is the master integrator of expression of MHC class II genes. It interacts with variety of basal transcription factors to initiate and elongate transcription of these genes. Among others, it recruits positive transcription elongation factor b (P-TEFb) to MHC class II promoters. In cells, P-TEFb is found in small active or large inactive complexes. The large complex is composed of P-TEFb, 7SK small nuclear RNA, and hexamethylene bisacetamide-inducible protein 1 (Hexim1). The present study identifies Hexim1 as a potent inhibitor of CIITA-mediated transcription. Not only the exogenously expressed but also IFN-gamma-induced CIITA was inhibited by Hexim1. This inhibition did riot result from an association between Hexim1 and CIITA but depended on the intact Cyclin T1-binding domain in Hexim1. importantly, Hexim1 sequestered P-TEFb from CIITA, as documented by binding competition and ChIP assays. Conversely, the depletion of Hexim1 from cells by siRNA increased CIITA-mediated transcription. Thus, modulating ratios between active and inactive P-TEFb complexes is an additional mechanism of regulating transcriptional activators such as CIITA.
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