| JAGOŠOVÁ, Jana, Lenka PITROVÁ, Jana SLOVÁČKOVÁ, Barbora RAVČUKOVÁ, Jan ŠMARDA and Jana ŠMARDOVÁ. Transactivation and reactivation capabilities of temperature-dependent p53 mutants in yeast and human cells. International Journal of Oncology. Athens, Greece: SPANDIDOS PUBL LTD, 2012, vol. 41, No 3, p. 1157-1163. ISSN 1019-6439. Available from: https://dx.doi.org/10.3892/ijo.2012.1520. |
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@article{987857,
author = {Jagošová, Jana and Pitrová, Lenka and Slováčková, Jana and Ravčuková, Barbora and Šmarda, Jan and Šmardová, Jana},
article_location = {Athens, Greece},
article_number = {3},
doi = {http://dx.doi.org/10.3892/ijo.2012.1520},
keywords = {p53; transactivation; temperature-dependent p53 mutants; discriminating p53 mutants; functional analysis of separated alleles in yeast},
language = {eng},
issn = {1019-6439},
journal = {International Journal of Oncology},
title = {Transactivation and reactivation capabilities of temperature-dependent p53 mutants in yeast and human cells},
volume = {41},
year = {2012}
}
TY - JOUR
ID - 987857
AU - Jagošová, Jana - Pitrová, Lenka - Slováčková, Jana - Ravčuková, Barbora - Šmarda, Jan - Šmardová, Jana
PY - 2012
TI - Transactivation and reactivation capabilities of temperature-dependent p53 mutants in yeast and human cells
JF - International Journal of Oncology
VL - 41
IS - 3
SP - 1157-1163
EP - 1157-1163
PB - SPANDIDOS PUBL LTD
SN - 10196439
KW - p53
KW - transactivation
KW - temperature-dependent p53 mutants
KW - discriminating p53 mutants
KW - functional analysis of separated alleles in yeast
N2 - The p53 protein is a sequence-specific transcription factor controlling the expression of multiple genes and protecting cells from oncogenic transformation. In many tumors, the p53 protein is completely or partially inactivated by mutations in the p53 gene. We analyzed the transactivating activity of nine human temperature-dependent (td) p53 mutants in yeast cells. Mutations in seven of them were localized in the beta-sandwich-coding region of the p53 gene, eight p53 mutants were temperature-sensitive and the R283C mutant was coldsensitive. Patterns of their transactivation abilities towards three different responsive elements, the extent of their temperature dependency as well as discriminativity, were considerably variable. Similarly, their capacity to become reactivated by amifostine varied from complete resistance to high sensitivity. Transactivation abilities and temperature dependency of six p53 td mutants were determined in transiently-transfected H1299 human cells and revealed substantial concordance between the activity patterns of the p53 mutants in yeast and human cells. We concluded that the td p53 mutants do not comprise a uniform group, therefore, the behavior of each mutant has to be tested individually.
ER -
JAGOŠOVÁ, Jana, Lenka PITROVÁ, Jana SLOVÁČKOVÁ, Barbora RAVČUKOVÁ, Jan ŠMARDA and Jana ŠMARDOVÁ. Transactivation and reactivation capabilities of temperature-dependent p53 mutants in yeast and human cells. \textit{International Journal of Oncology}. Athens, Greece: SPANDIDOS PUBL LTD, 2012, vol.~41, No~3, p.~1157-1163. ISSN~1019-6439. Available from: https://dx.doi.org/10.3892/ijo.2012.1520.
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