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@article{987912, author = {Doležalová, Dáša and Mráz, Marek and Bárta, Tomáš and Plevová, Karla and Vinarský, Vladimír and Holubcová, Zuzana and Jaroš, Josef and Dvořák, Petr and Pospíšilová, Šárka and Hampl, Aleš}, article_location = {Miamisburg, Ohio}, article_number = {7}, doi = {http://dx.doi.org/10.1002/stem.1108}, keywords = {Human embryonic stem cells; microRNA; DNA damage; p21; CDKN1A; p53; miR-302}, language = {eng}, issn = {1066-5099}, journal = {Stem Cells}, title = {MicroRNAs Regulate p21(Waf1/Cip1) Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells}, volume = {30}, year = {2012} }
TY - JOUR ID - 987912 AU - Doležalová, Dáša - Mráz, Marek - Bárta, Tomáš - Plevová, Karla - Vinarský, Vladimír - Holubcová, Zuzana - Jaroš, Josef - Dvořák, Petr - Pospíšilová, Šárka - Hampl, Aleš PY - 2012 TI - MicroRNAs Regulate p21(Waf1/Cip1) Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells JF - Stem Cells VL - 30 IS - 7 SP - 1362-1372 EP - 1362-1372 PB - AlphaMed Press SN - 10665099 KW - Human embryonic stem cells KW - microRNA KW - DNA damage KW - p21 KW - CDKN1A KW - p53 KW - miR-302 N2 - Studies of human embryonic stem cells (hESCs) commonly describe the nonfunctional p53-p21 axis of the G1/S checkpoint pathway with subsequent relevance for cell cycle regulation and the DNA damage response (DDR). Importantly, p21 mRNA is clearly present and upregulated after the DDR in hESCs, but p21 protein is not detectable. In this article, we provide evidence that expression of p21 protein is directly regulated by the microRNA (miRNA) pathway under standard culture conditions and after DNA damage. The DDR in hESCs leads to upregulation of tens of miRNAs, including hESC-specific miRNAs such as those of the miR-302 family, miR-371-372 family, or C19MC miRNA cluster. Most importantly, we show that the hESC-enriched miRNA family miR-302 (miR-302a, miR-302b, miR-302c, and miR-302d) directly contributes to regulation of p21 expression in hESCs and, thus, demonstrate a novel function for miR-302s in hESCS. The described mechanism elucidates the role of miRNAs in regulation of important molecular pathway governing the G1/S transition checkpoint before as well as after DNA damage. STEM CELLS 2012;30:1362-1372 ER -
DOLEŽALOVÁ, Dáša, Marek MRÁZ, Tomáš BÁRTA, Karla PLEVOVÁ, Vladimír VINARSKÝ, Zuzana HOLUBCOVÁ, Josef JAROŠ, Petr DVOŘÁK, Šárka POSPÍŠILOVÁ a Aleš HAMPL. MicroRNAs Regulate p21(Waf1/Cip1) Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells. Online. \textit{Stem Cells}. Miamisburg, Ohio: AlphaMed Press, 2012, roč.~30, č.~7, s.~1362-1372. ISSN~1066-5099. Dostupné z: https://dx.doi.org/10.1002/stem.1108. [citováno 2024-04-24]
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