DOLEŽALOVÁ, Dáša, Marek MRÁZ, Tomáš BÁRTA, Karla PLEVOVÁ, Vladimír VINARSKÝ, Zuzana HOLUBCOVÁ, Josef JAROŠ, Petr DVOŘÁK, Šárka POSPÍŠILOVÁ and Aleš HAMPL. MicroRNAs Regulate p21(Waf1/Cip1) Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells. Stem Cells. Miamisburg, Ohio: AlphaMed Press, 2012, vol. 30, No 7, p. 1362-1372. ISSN 1066-5099. doi:10.1002/stem.1108.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name MicroRNAs Regulate p21(Waf1/Cip1) Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells
Authors DOLEŽALOVÁ, Dáša (703 Slovakia, belonging to the institution), Marek MRÁZ (203 Czech Republic, belonging to the institution), Tomáš BÁRTA (203 Czech Republic, belonging to the institution), Karla PLEVOVÁ (203 Czech Republic, belonging to the institution), Vladimír VINARSKÝ (203 Czech Republic, belonging to the institution), Zuzana HOLUBCOVÁ (203 Czech Republic, belonging to the institution), Josef JAROŠ (203 Czech Republic, belonging to the institution), Petr DVOŘÁK (203 Czech Republic, belonging to the institution), Šárka POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution) and Aleš HAMPL (203 Czech Republic, guarantor, belonging to the institution).
Edition Stem Cells, Miamisburg, Ohio, AlphaMed Press, 2012, 1066-5099.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 7.701
RIV identification code RIV/00216224:14110/12:00057498
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1002/stem.1108
UT WoS 000305477000005
Keywords in English Human embryonic stem cells; microRNA; DNA damage; p21; CDKN1A; p53; miR-302
Tags International impact
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 13. 2. 2013 22:06.
Abstract
Studies of human embryonic stem cells (hESCs) commonly describe the nonfunctional p53-p21 axis of the G1/S checkpoint pathway with subsequent relevance for cell cycle regulation and the DNA damage response (DDR). Importantly, p21 mRNA is clearly present and upregulated after the DDR in hESCs, but p21 protein is not detectable. In this article, we provide evidence that expression of p21 protein is directly regulated by the microRNA (miRNA) pathway under standard culture conditions and after DNA damage. The DDR in hESCs leads to upregulation of tens of miRNAs, including hESC-specific miRNAs such as those of the miR-302 family, miR-371-372 family, or C19MC miRNA cluster. Most importantly, we show that the hESC-enriched miRNA family miR-302 (miR-302a, miR-302b, miR-302c, and miR-302d) directly contributes to regulation of p21 expression in hESCs and, thus, demonstrate a novel function for miR-302s in hESCS. The described mechanism elucidates the role of miRNAs in regulation of important molecular pathway governing the G1/S transition checkpoint before as well as after DNA damage. STEM CELLS 2012;30:1362-1372
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GAP301/10/1971, research and development projectName: Exprese, signalizace a funkce receptorů smrti v lidských embryonálních kmenových buňkách.
Investor: Czech Science Foundation, Standard Projects
MSM0021622430, plan (intention)Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie
Investor: Ministry of Education, Youth and Sports of the CR, Research Intents
NT11218, research and development projectName: Funkční a strukturní změny microRNA u lymfoproliferativních malignit a jejich vliv na prognózu onemocnění a predikci léčebné odpovědi
Type Name Uploaded/Created by Uploaded/Created Rights
Dolezalova_Stem_Cells_2012.pdf   File version Petr, M. 30. 7. 2012

Rights

Right to read
 
Right to upload
 
Right to administer:
  • a concrete person Ing. Josef Jaroš, Ph.D., učo 100474
  • a concrete person doc. MUDr. Mgr. Marek Mráz, Ph.D., učo 101627
  • a concrete person Mgr. Tomáš Bárta, Ph.D., učo 106061
  • a concrete person Mgr. Karla Plevová, Ph.D., učo 106100
  • a concrete person Mgr. Vladimír Vinarský, Ph.D., učo 106932
  • a concrete person Mgr. Dáša Bohačiaková, Ph.D., učo 123980
  • a concrete person prof. RNDr. Šárka Pospíšilová, Ph.D., učo 38791
  • a concrete person doc. MVDr. Aleš Hampl, CSc., učo 47170
  • a concrete person prof. Ing. Petr Dvořák, CSc., učo 47260
  • a concrete person PharmDr. Zuzana Holubcová, Ph.D., učo 59820
  • a concrete person Mgr. Michal Petr, učo 65024
Attributes
 
Print
Ask the author for author copy Displayed: 5. 8. 2021 20:54