Detailed Information on Publication Record

KOPECKÁ, Marie, S. YOSHIDA and M. YAMAGUCHI. Actin ring formation around the cell nucleus of long-neck yeast. Journal of Electron Microscopy. Tokyo, 2012, vol. 61, No 4, p. 249-255. ISSN 0022-0744. Available from: https://dx.doi.org/10.1093/jmicro/dfs049.

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Basic information
Original name	Actin ring formation around the cell nucleus of long-neck yeast
Authors	KOPECKÁ, Marie (203 Czech Republic, guarantor, belonging to the institution), S. YOSHIDA (392 Japan) and M. YAMAGUCHI (392 Japan).
Edition	Journal of Electron Microscopy, Tokyo, 2012, 0022-0744.

Other information
Original language	English
Type of outcome	Article in a journal
Field of Study	10600 1.6 Biological sciences
Country of publisher	Japan
Confidentiality degree	is not subject to a state or trade secret
Impact factor	Impact factor: 1.438
RIV identification code	RIV/00216224:14110/12:00060492
Organization unit	Faculty of Medicine
Doi	http://dx.doi.org/10.1093/jmicro/dfs049
UT WoS	000307499800007
Keywords in English	F-actin; yeast; nucleus; perinuclear actin ring
Tags	International impact
Changed by	Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 13/2/2013 17:54.

Abstract
The unique long-neck yeast Fellomyces fuzhouensis has F-actin cables and cortical patches. Here, we describe a new F-actin structure present in fungi, a perinuclear F-actin collar ring around the cell nucleus. This F-actin structure can be visualized by fluorescent microscopic imaging of rhodamine–phalloidin-stained F-actin in cells treated with the mitotic drug isopropyl N-(3-chlorophenyl) carbamate or the microtubule inhibitor thiabendazol or when cells were grown in cut dried radish medium or yeast extract pepton dextrose (YEPD) medium. In contrast, these structures were absent in cells treated with Latrunculin A. The hypothetical functions of the F-actin ring are discussed.

Abstract

The unique long-neck yeast Fellomyces fuzhouensis has F-actin cables and cortical patches. Here, we describe a new F-actin structure present in fungi, a perinuclear F-actin collar ring around the cell nucleus. This F-actin structure can be visualized by fluorescent microscopic imaging of rhodamine–phalloidin-stained F-actin in cells treated with the mitotic drug isopropyl N-(3-chlorophenyl) carbamate or the microtubule inhibitor thiabendazol or when cells were grown in cut dried radish medium or yeast extract pepton dextrose (YEPD) medium. In contrast, these structures were absent in cells treated with Latrunculin A. The hypothetical functions of the F-actin ring are discussed.