PORRUA-FUERTE, Odil, Fruzsina HÓBOR, Jocelyne BOULAY, Karel KUBÍČEK, Yves D’AUBENTON-CARAFA, Rajani Kanth GUDIPATI, Richard ŠTEFL and Domenico LIBRI. In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination. EMBO Journal. NEW YORK: NATURE PUBLISHING GROUP, 2012, vol. 31, No 19, p. 3935-3948. ISSN 0261-4189. Available from: https://dx.doi.org/10.1038/emboj.2012.237. |
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@article{988334, author = {PorruaandFuerte, Odil and Hóbor, Fruzsina and Boulay, Jocelyne and Kubíček, Karel and D’AubentonandCarafa, Yves and Gudipati, Rajani Kanth and Štefl, Richard and Libri, Domenico}, article_location = {NEW YORK}, article_number = {19}, doi = {http://dx.doi.org/10.1038/emboj.2012.237}, keywords = {cryptic unstable transcripts; transcription termination; Nrd1p-Nab3p-Sen1p complex; hidden transcription; transcriptional insulators}, language = {eng}, issn = {0261-4189}, journal = {EMBO Journal}, title = {In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination}, url = {http://www.nature.com/emboj/journal/v31/n19/abs/emboj2012237a.html}, volume = {31}, year = {2012} }
TY - JOUR ID - 988334 AU - Porrua-Fuerte, Odil - Hóbor, Fruzsina - Boulay, Jocelyne - Kubíček, Karel - D’Aubenton-Carafa, Yves - Gudipati, Rajani Kanth - Štefl, Richard - Libri, Domenico PY - 2012 TI - In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination JF - EMBO Journal VL - 31 IS - 19 SP - 3935-3948 EP - 3935-3948 PB - NATURE PUBLISHING GROUP SN - 02614189 KW - cryptic unstable transcripts KW - transcription termination KW - Nrd1p-Nab3p-Sen1p complex KW - hidden transcription KW - transcriptional insulators UR - http://www.nature.com/emboj/journal/v31/n19/abs/emboj2012237a.html L2 - http://www.nature.com/emboj/journal/v31/n19/abs/emboj2012237a.html N2 - The Nrd1-Nab3-Sen1 (NNS) complex pathway is responsible for transcription termination of cryptic unstable transcripts and sn/snoRNAs. The NNS complex recognizes short motifs on the nascent RNA, but the presence of these sequences alone is not sufficient to define a functional terminator. We generated a homogeneous set of several hundreds of artificial, NNS-dependent terminators with an in vivo selection approach. Analysis of these terminators revealed novel and extended sequence determinants for transcription termination and NNS complex binding as well as supermotifs that are critical for termination. Biochemical and structural data revealed that affinity and specificity of RNA recognition by Nab3p relies on induced fit recognition implicating an a-helical extension of the RNA recognition motif. Interestingly, the same motifs can be recognized by the NNS or the mRNA termination complex depending on their position relative to the start of transcription, suggesting that they function as general transcriptional insulators to prevent interference between the non-coding and the coding yeast transcriptomes. ER -
PORRUA-FUERTE, Odil, Fruzsina HÓBOR, Jocelyne BOULAY, Karel KUBÍČEK, Yves D’AUBENTON-CARAFA, Rajani Kanth GUDIPATI, Richard ŠTEFL and Domenico LIBRI. In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination. \textit{EMBO Journal}. NEW YORK: NATURE PUBLISHING GROUP, 2012, vol.~31, No~19, p.~3935-3948. ISSN~0261-4189. Available from: https://dx.doi.org/10.1038/emboj.2012.237.
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