J 2012

Human Escherichia coli strains of different geographical and time source: bacteriocin types and their gene sequences are population-specific

ŠMAJS, David, Darina ČEJKOVÁ, Lenka MICENKOVÁ, Claudia I. LIMA-BITTENCOURT, Edmar CHARTONE-SOUZA et. al.

Basic information

Original name

Human Escherichia coli strains of different geographical and time source: bacteriocin types and their gene sequences are population-specific

Authors

ŠMAJS, David (203 Czech Republic, guarantor, belonging to the institution), Darina ČEJKOVÁ (203 Czech Republic, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Claudia I. LIMA-BITTENCOURT (76 Brazil), Edmar CHARTONE-SOUZA (76 Brazil), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and Andréa M. A. NASCIMENTO (76 Brazil)

Edition

ENVIRONMENTAL MICROBIOLOGY REPORTS, 2012, 1758-2229

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.708

RIV identification code

RIV/00216224:14110/12:00060540

Organization unit

Faculty of Medicine

DOI

UT WoS

000306404600013

Keywords in English

PLASMIDS; COLICINOGENY; DIVERSITY; FREQUENCY; NISSLE; TRACT; PIGS; H22; E1

Tags

International impact
Změněno: 22/4/2013 14:59, Soňa Böhmová

Abstract

V originále

Bacteriocin production was tested in two sets of Escherichia coli strains: one isolated in 1978 from healthy children of rubber tree tappers and Indians in Amazonia, Brazil, and the second one isolated in 2004 from healthy adult volunteers in the Moravia region, Czech Republic. Although the occurrence of bacteriocin production was rather similar in both groups (54.3% and 43.4% respectively), the spectra of bacteriocin-encoding determinants in both groups were different. Altogether, 12 different bacteriocin-encoding determinants were found among the tested strains. The occurrence of colicin E1- and Y-genes was higher while the occurrence of microcin V gene was lower among Amazonian strains compared with Moravian strains. The colicin-encoding determinants of colicins Ia, M, Y and E1 were amplified from Amazonian and Moravian producer strains and sequenced, as were chromosomal 16S rRNA, gyrB and tonB genes. While sequence alignments of chromosomal loci revealed no clusters with respect to geographical origin of strains, the colicin-encoding genes were very similar among the strains of each origin but different between the two geographic groups.

Links

MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
MUNI/A/0822/2011, interní kód MU
Name: Molekulární a buněčná biologie v biomedicíně
Investor: Masaryk University, Category A
NS9665, research and development project
Name: Molekulární typizace bakteriocinů produkovaných kmeny Escherichia coli z klinického materiálu: identifikace kolicinů a mikrocinů v souborech komensálních a patogenních kmenů
Investor: Ministry of Health of the CR

Files attached

emi4365.pdf
Request the author's version of the file