KYPR, Jaroslav, Iva KEJNOVSKÁ, Klára BEDNÁŘOVÁ a Michaela VORLÍČKOVÁ. CIRCULAR DICHROISM SPECTROSCOPY OF NUCLEIC ACIDS. In Berova, N., Polavarapu, L. P., Nakanishi, K., Woody, R.W. Comprehensive Chiroptical Spectroscopy : Applications in Stereochemical Analysis of Synthetic Compounds, Natural Products, and Biomolecules. Hoboken (NJ, USA): John Wiley & Sons, 2012. s. 575-586. Volume 2. ISBN 978-1-118-01292-5. doi:10.1002/9781118120392.ch17.
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Základní údaje
Originální název CIRCULAR DICHROISM SPECTROSCOPY OF NUCLEIC ACIDS
Autoři KYPR, Jaroslav, Iva KEJNOVSKÁ, Klára BEDNÁŘOVÁ a Michaela VORLÍČKOVÁ.
Vydání Hoboken (NJ, USA), Comprehensive Chiroptical Spectroscopy : Applications in Stereochemical Analysis of Synthetic Compounds, Natural Products, and Biomolecules, od s. 575-586, 12 s. Volume 2, 2012.
Nakladatel John Wiley & Sons
Další údaje
Originální jazyk angličtina
Typ výsledku Kapitola resp. kapitoly v odborné knize
Obor 10610 Biophysics
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
Forma vydání tištěná verze "print"
WWW URL
Organizační jednotka Středoevropský technologický institut
ISBN 978-1-118-01292-5
Doi http://dx.doi.org/10.1002/9781118120392.ch17
Klíčová slova anglicky nucleic acid CD spectroscopy; guanine quadruplexes; CD spectra; whole spectrum; CD; interpretation
Štítky ne MU, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Olga Křížová, učo 56639. Změněno: 11. 4. 2013 10:58.
Anotace
Circular dichroism (CD) arises from differential absorption of right-handed and left-handed circularly polarized light by chiral molecules. In nucleic acids there are three sources of chirality. First is the asymmetric sugar; this chirality causes monomeric nucleosides to exhibit CD. The second source is the helicity of the secondary structures adopted by nucleic acids. The third source of CD results from long-range tertiary ordering of DNA in some environments. CD of monomeric constituents of nucleic acids and short single-stranded fragments were described previously. The theory of CD is well-developed and complex. Nevertheless, the use of CD spectroscopy to elucidate nucleic acid secondary structure is mainly based on empirical grounds. Conventional CD spectroscopy operates within the spectral range of about 200 nm to 320 nm. For these measurements, conventional spectrometers are used. CD spectroscopy is even more sensitive and informative in the far UV region below 200 nm, but these measurements are difficult to perform and the specialized instruments required are expensive. CD spectra of nucleic acids can also be measured in the infrared region, but here the method is much less sensitive. In this chapter we will focus on CD results obtained in the 200- to 320-nm range, the range mostly used to study secondary structures of nucleic acids.
Návaznosti
ED1.1.00/02.0068, projekt VaVNázev: CEITEC - central european institute of technology
VytisknoutZobrazeno: 2. 12. 2022 00:40