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@article{988812,
author = {Divišová, Šárka and Dubový, Petr and Vlčková, Eva and Němec, Martin and Klusáková, Ilona and Bělobrádková, Jana and Bednařík, Josef},
article_number = {1-2},
doi = {http://dx.doi.org/10.1016/j.jns.2011.11.038},
keywords = {Growth-associated protein 43; protein gene product 9.5; skin biopsy; type 2 diabetes mellitus; diabetic neuropathy; regeneration},
language = {eng},
issn = {0022-510X},
journal = {Journal of Neurological Sciences},
title = {Expression of growth-associated protein 43 in the skin nerve fibers of patients with type 2 diabetes mellitus},
url = {http://www.ncbi.nlm.nih.gov/pubmed/22209024},
volume = {315},
year = {2012}
}
TY - JOUR
ID - 988812
AU - Divišová, Šárka - Dubový, Petr - Vlčková, Eva - Němec, Martin - Klusáková, Ilona - Bělobrádková, Jana - Bednařík, Josef
PY - 2012
TI - Expression of growth-associated protein 43 in the skin nerve fibers of patients with type 2 diabetes mellitus
JF - Journal of Neurological Sciences
VL - 315
IS - 1-2
SP - 60-63
EP - 60-63
PB - Elsevier Science
SN - 0022510X
KW - Growth-associated protein 43
KW - protein gene product 9.5
KW - skin biopsy
KW - type 2 diabetes mellitus
KW - diabetic neuropathy
KW - regeneration
UR - http://www.ncbi.nlm.nih.gov/pubmed/22209024
L2 - http://www.ncbi.nlm.nih.gov/pubmed/22209024
N2 - The growth-associated protein 43 (GAP-43) is known as a marker of regenerating nerve fibers and their continuous remodeling in the adult human skin. The purpose of this pilot study was to investigate a possible role for GAP-43 in the detection of the early stages of small-fiber neuropathy in patients with type 2 diabetes mellitus (DM2) as compared with a well- established and validated parameter – intra-epidermal nerve fiber density (IENFD) of protein gene product 9.5 (PGP 9.5) immunoreactive intra-epidermal C fibers. In a group of 21 patients with DM2 within three years of diagnosis (13 men, 8 women; mean age 53.9 +- 12.8; range 30 – 74) and a group of 17 healthy volunteers (8 men, 9 women; mean age 55.8 +- 8.5; range 45 – 70 years), skin punch biopsies were taken from a distal calf and double immunostained with both PGP 9.5 and GAP-43. In healthy controls, 96.8% of 629 PGP 9.5 immunoreactive fibers were immunostained with GAP-43; the proportion of PGP 9.5 intra-epidermal nerve fibers immunoreactive for GAP-43 in control subjects ranged from 86.5 to 100%. In DM2 patients, IENFD was significantly lower compared to controls (median, 1.5 vs. 11.2/mm; p <0.001). The proportion of GAP-43 immunoreactive intraepidermal nerve fibers was significantly lower in DM2 patients compared to healthy controls (73.6% of 337 PGP 9.5 positive fibers; p <0.001); ranged from 0 to 98.1%. In conclusion, these results show that impaired regeneration of intra-epidermal C fibers in the early stages of type 2 diabetes mellitus, as indicated by GAP-43, might be a marker of incipient diabetic neuropathy.
ER -
DIVIŠOVÁ, Šárka, Petr DUBOVÝ, Eva VLČKOVÁ, Martin NĚMEC, Ilona KLUSÁKOVÁ, Jana BĚLOBRÁDKOVÁ and Josef BEDNAŘÍK. Expression of growth-associated protein 43 in the skin nerve fibers of patients with type 2 diabetes mellitus. \textit{Journal of Neurological Sciences}. Elsevier Science, 2012, vol.~315, 1-2, p.~60-63. ISSN~0022-510X. Available from: https://dx.doi.org/10.1016/j.jns.2011.11.038.
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