VORLÍČKOVÁ, Michaela, Iva KEJNOVSKÁ, Janos SAGI, Daniel RENČIUK, Klára BEDNÁŘOVÁ, Jitka MOTLOVÁ and Jaroslav KYPR. Circular dichroism and guanine quadruplexes. Methods. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2012, vol. 57, No 1, p. 64-75. ISSN 1046-2023. doi:10.1016/j.ymeth.2012.03.011.
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Basic information
Original name Circular dichroism and guanine quadruplexes
Authors VORLÍČKOVÁ, Michaela (203 Czech Republic, guarantor, belonging to the institution), Iva KEJNOVSKÁ (203 Czech Republic), Janos SAGI (348 Hungary), Daniel RENČIUK (203 Czech Republic, belonging to the institution), Klára BEDNÁŘOVÁ (203 Czech Republic), Jitka MOTLOVÁ (203 Czech Republic) and Jaroslav KYPR (203 Czech Republic, belonging to the institution).
Edition Methods, SAN DIEGO, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2012, 1046-2023.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.641
RIV identification code RIV/00216224:14740/12:00057102
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.ymeth.2012.03.011
UT WoS 000307912100008
Keywords in English Polymorphism of quadruplex arrangements; DNA; RNA; CD spectra and structure of nucleic acids; Duplexes and tetraplexes; Cooperative and non-cooperative DNA transitions; Guanine-rich genomic sequences
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 23. 4. 2013 07:39.
Abstract
Circular dichroism (CD) is remarkably sensitive to the conformational states of nucleic acids; therefore, CD spectroscopy has been used to study most features of DNA and RNA structures. Quadruplexes are among the significant noncanonical nucleic acids architectures that have received special attentions recently. This article presents examples on the contribution of CD spectroscopy to our knowledge of quadruplex structures and their polymorphism. The examples were selected to demonstrate the potential of this simple method in the quadruplex field. As CD spectroscopy detects only the global feature of a macromolecule, it should preferably be used in combination with other techniques. On the other hand, CD spectroscopy, often as a pioneering approach, can reveal the formation of particular structural arrangements, to search for the conditions stabilizing the structures, to follow the transitions between various structural states, to explore kinetics of their appearance, to determine thermodynamic parameters and also detect formation of higher order structures. This article aims to show that CD spectroscopy is an important complementary technique to NMR spectroscopy and X-ray diffraction in quadruplex studies.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
IAA500040903, research and development projectName: Biofyzika a bioinformatika genomových fragmentů DNA velice bohatých na guanin a adenin
Investor: Academy of Sciences of the Czech Republic, Biophysics and bioinformatics of genomic DNA fragments very rich in guanine and adenine
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