HOFMANOVA, Jirina, Miroslav CIGANEK, Josef SLAVIK, Alois KOZUBIK, Lenka STIXOVA, Alena VACULOVÁ, Ladislav DUŠEK a Miroslav MACHALA. Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids. JOURNAL OF NUTRITIONAL BIOCHEMISTRY. NEW YORK: ELSEVIER SCIENCE INC, 2012, roč. 23, č. 6, s. 539-548. ISSN 0955-2863. doi:10.1016/j.jnutbio.2011.02.010.
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Základní údaje
Originální název Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids
Autoři HOFMANOVA, Jirina (203 Česká republika, garant), Miroslav CIGANEK (203 Česká republika), Josef SLAVIK (203 Česká republika), Alois KOZUBIK (203 Česká republika), Lenka STIXOVA (203 Česká republika), Alena VACULOVÁ (203 Česká republika), Ladislav DUŠEK (203 Česká republika, domácí) a Miroslav MACHALA (203 Česká republika).
Vydání JOURNAL OF NUTRITIONAL BIOCHEMISTRY, NEW YORK, ELSEVIER SCIENCE INC, 2012, 0955-2863.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10600 1.6 Biological sciences
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 4.552
Kód RIV RIV/00216224:14110/12:00060589
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.1016/j.jnutbio.2011.02.010
UT WoS 000304786000004
Klíčová slova anglicky Colon cancer; Polyunsaturated fatty acids; Butyrate; Lipid analyses; Apoptosis; Differentiation
Příznaky Mezinárodní význam
Změnil Změnil: Mgr. Michal Petr, učo 65024. Změněno: 15. 8. 2012 09:26.
Anotace
The present study highlights the important association between lipid alterations and differentiation/apoptotic responses in human colon differentiating (FHC) and nondifferentiating (HCT-116) cell lines after their treatment with short-chain fatty acid sodium butyrate (NaBt), polyunsaturated fatty acids (PUFAs), and/or their combination. Our data from GC/MS and LC/MS/MS showed an effective incorporation and metabolization of the supplemented arachidonic acid (AA) or docosahexaenoic acid (DNA), resulting in an enhanced content of the respective PUFA in individual phospholipid (PL) classes and an altered composition of the whole cellular fatty acid spectrum in both FHC and HCT-116 cells. We provide novel evidence that NaBt combined with PUFAs additionally modulated AA and DHA cellular levels and caused their shift from triacylglycerol to PL fractions. NaBt increased, while AA, DHA and their combination with NaBt decreased endogenous fatty acid synthesis in FHC but not in HCT-116 cells. Fatty acid treatment also altered membrane lipid structure, augmented cytoplasmic lipid droplet accumulation, reactive oxygen species (ROS) production and dissipation of the mitochondrial membrane potential. All these parameters were significantly enhanced by combined NaBt/PUFA treatment, but only in FHC cells was this accompanied by highly increased apoptosis and suppressed differentiation. Moreover, the most significant changes of ROS production, differentiation and apoptosis among the parameters studied, the highest effects of combined NaBt/PUFA treatment and a lower sensitivity of HCT-116 cells were confirmed using two-way ANOVA. Our results demonstrate an important role of fatty acid-induced lipid alterations in the different apoptotic/differentiation response of colon cells with various carcinogenic potential. (C) 2012 Elsevier Inc. All rights reserved.
VytisknoutZobrazeno: 23. 5. 2022 04:58