HOFMANOVA, Jirina, Miroslav CIGANEK, Josef SLAVIK, Alois KOZUBIK, Lenka STIXOVA, Alena VACULOVÁ, Ladislav DUŠEK and Miroslav MACHALA. Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids. JOURNAL OF NUTRITIONAL BIOCHEMISTRY. NEW YORK: ELSEVIER SCIENCE INC, 2012, vol. 23, No 6, p. 539-548. ISSN 0955-2863. doi:10.1016/j.jnutbio.2011.02.010.
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Basic information
Original name Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids
Authors HOFMANOVA, Jirina (203 Czech Republic, guarantor), Miroslav CIGANEK (203 Czech Republic), Josef SLAVIK (203 Czech Republic), Alois KOZUBIK (203 Czech Republic), Lenka STIXOVA (203 Czech Republic), Alena VACULOVÁ (203 Czech Republic), Ladislav DUŠEK (203 Czech Republic, belonging to the institution) and Miroslav MACHALA (203 Czech Republic).
Edition JOURNAL OF NUTRITIONAL BIOCHEMISTRY, NEW YORK, ELSEVIER SCIENCE INC, 2012, 0955-2863.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 4.552
RIV identification code RIV/00216224:14110/12:00060589
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1016/j.jnutbio.2011.02.010
UT WoS 000304786000004
Keywords in English Colon cancer; Polyunsaturated fatty acids; Butyrate; Lipid analyses; Apoptosis; Differentiation
Tags International impact
Changed by Changed by: Mgr. Michal Petr, učo 65024. Changed: 15. 8. 2012 09:26.
Abstract
The present study highlights the important association between lipid alterations and differentiation/apoptotic responses in human colon differentiating (FHC) and nondifferentiating (HCT-116) cell lines after their treatment with short-chain fatty acid sodium butyrate (NaBt), polyunsaturated fatty acids (PUFAs), and/or their combination. Our data from GC/MS and LC/MS/MS showed an effective incorporation and metabolization of the supplemented arachidonic acid (AA) or docosahexaenoic acid (DNA), resulting in an enhanced content of the respective PUFA in individual phospholipid (PL) classes and an altered composition of the whole cellular fatty acid spectrum in both FHC and HCT-116 cells. We provide novel evidence that NaBt combined with PUFAs additionally modulated AA and DHA cellular levels and caused their shift from triacylglycerol to PL fractions. NaBt increased, while AA, DHA and their combination with NaBt decreased endogenous fatty acid synthesis in FHC but not in HCT-116 cells. Fatty acid treatment also altered membrane lipid structure, augmented cytoplasmic lipid droplet accumulation, reactive oxygen species (ROS) production and dissipation of the mitochondrial membrane potential. All these parameters were significantly enhanced by combined NaBt/PUFA treatment, but only in FHC cells was this accompanied by highly increased apoptosis and suppressed differentiation. Moreover, the most significant changes of ROS production, differentiation and apoptosis among the parameters studied, the highest effects of combined NaBt/PUFA treatment and a lower sensitivity of HCT-116 cells were confirmed using two-way ANOVA. Our results demonstrate an important role of fatty acid-induced lipid alterations in the different apoptotic/differentiation response of colon cells with various carcinogenic potential. (C) 2012 Elsevier Inc. All rights reserved.
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