Detailed Information on Publication Record
2012
Adipophilin (ADRP/ADPH/PLIN2): Variability in Exonic Regions and Secretion of Adipophilin into Breast Milk
ŠPLÍCHAL, Zbyněk, Laëtitia FAUVEAU, Julie BIENERTOVÁ VAŠKŮ, Josef TOMANDL, Marie TOMANDLOVÁ et. al.Basic information
Original name
Adipophilin (ADRP/ADPH/PLIN2): Variability in Exonic Regions and Secretion of Adipophilin into Breast Milk
Name in Czech
Adipofilin (ADRP/ADPH/PLIN2): variabilita v exonických oblastech v souvislosti se sekrecí adipofilinu do mateřského mléka
Authors
ŠPLÍCHAL, Zbyněk (203 Czech Republic), Laëtitia FAUVEAU (250 France), Julie BIENERTOVÁ VAŠKŮ (203 Czech Republic, guarantor, belonging to the institution), Josef TOMANDL (203 Czech Republic), Marie TOMANDLOVÁ (203 Czech Republic), Zuzana HODICKÁ (203 Czech Republic), Pavel VENTRUBA (203 Czech Republic), Anna VAŠKŮ (203 Czech Republic) and Petr BIENERT (203 Czech Republic)
Edition
European Human Genetics Conference 2012, 2012
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
30214 Obstetrics and gynaecology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/12:00060618
Organization unit
Faculty of Medicine
Keywords (in Czech)
adipofilin; kojení; mateřské mléko
Keywords in English
adipophilin; breastfeeding; breast milk
Tags
International impact, Reviewed
Změněno: 25/3/2013 09:25, Ing. Mgr. Věra Pospíšilíková
V originále
Introduction: Adipophilin (ADRP/ADPH/PLIN2), a member of the perilipin (PAT) family of lipid droplet proteins, is believed to play a crucial role in both formation and secretion of milk lipids in mammals. The aim of our study was to determine whether adipophilin is secreted into human breast milk and to associate adipophilin levels in breast milk with the exonic variations of the adipophilin gene. Material and methods: The total of 20 pregnant women with physiological pregnancy, originating from the Caucasian Central-European population, were enrolled into the study and serum-milk sample duos were collected at the time of birth and at the days 1-3, 12-14, 28-30, 88-90 and 178-180 postpartum and investigated using ELISA-based methodology. The exons 3-7 of the PLIN2 gene were directly sequenced.Results: Adipophilin was constantly secreted into breast milk during the whole period of the study. The maternal serum circulating levels were extremely low (<0.8 ug/l), while the adipophilin levels in breast milk substantially exceeded serum levels at the given timepoints (p = 0.03). Two SNPs in exonic sequence of PLIN2 gene were identified, synonymous rs2228416 and missense rs35568725 that were not associated with maternal serum / breast milk levels of adipophilin. Conclusion: This is the first study to demonstrate that adipophilin is secreted into human breast milk during the whole 6 months after the birth. We do not report major association of investigated exonic variations of PLIN2 gene with adipophilin levels in maternal serum / breast milk in the Central- European population.
In Czech
Jedná se o první studii prokazující, že adipofilin je u člověka secernován do mateřského mléka, nepozorovali jsme významnou souvislosti mezi nalezenými SNP v exonických oblastech genu pro adipofilin a jeho sekrecí do mléka.
Links
| MUNI/A/0831/2011, interní kód MU |
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