VAŘECHA, Miroslav, Daniela PÁCLOVÁ, Jiřina PROCHÁZKOVÁ, Pavel MATULA, Dušan CMARKO and Michal KOZUBEK. Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I. BIOCELL. Argentina: National Council of Scientific and Techn, 2012, vol. 36, No 3, p. 121-126. ISSN 0327-9545.
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Basic information
Original name Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I
Name in Czech Snížení množství apoptózu vyvolávajícího faktoru narušuje funkci dýchacího komplexu I
Authors VAŘECHA, Miroslav (203 Czech Republic, guarantor, belonging to the institution), Daniela PÁCLOVÁ (203 Czech Republic, belonging to the institution), Jiřina PROCHÁZKOVÁ (203 Czech Republic, belonging to the institution), Pavel MATULA (203 Czech Republic, belonging to the institution), Dušan CMARKO (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic, belonging to the institution).
Edition BIOCELL, Argentina, National Council of Scientific and Techn, 2012, 0327-9545.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Argentina
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.575
RIV identification code RIV/00216224:14330/12:00057522
Organization unit Faculty of Informatics
UT WoS 000328358900004
Keywords (in Czech) superoxid;fluorescenční mikroskopie;obrazová analýza;mitochondrie;telomeráza
Keywords in English superoxide;fluorescence microscopy;image analysis;mitochondria;telomerase
Tags International impact, Reviewed
Changed by Changed by: Mgr. Miroslav Vařecha, Ph.D., učo 82780. Changed: 20. 2. 2013 09:59.
Abstract
Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Our results show that tumoral telomerase-positive (TP) AIF knockdown cell lines display significant increase in superoxide production in comparison to control cells, while a non-tumoral cell line and tumoral telomerase-negative cell lines with alternative lengthening of telomeres (ALT) show a decrease in superoxide production. According to these results, we can conclude that AIF knockdown disrupts function of complex I and therefore increases the superoxide production in mitochondria. The distinct effect of AIF depletion in various cell lines could result from recently discovered activity of telomerase in mitochondria of TP cancer cells, but this hypothesis needs further investigation.
Links
GBP302/12/G157, research and development projectName: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation
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