| VAŘECHA, Miroslav, Daniela PÁCLOVÁ, Jiřina PROCHÁZKOVÁ, Pavel MATULA, Dušan CMARKO and Michal KOZUBEK. Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I. BIOCELL. Argentina: National Council of Scientific and Techn, 2012, vol. 36, No 3, p. 121-126. ISSN 0327-9545. |
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@article{989665,
author = {Vařecha, Miroslav and Páclová, Daniela and Procházková, Jiřina and Matula, Pavel and Cmarko, Dušan and Kozubek, Michal},
article_location = {Argentina},
article_number = {3},
keywords = {superoxide;fluorescence microscopy;image analysis;mitochondria;telomerase},
language = {eng},
issn = {0327-9545},
journal = {BIOCELL},
note = {Accepted.},
title = {Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I},
url = {http://www.scielo.org.ar/scielo.php?script=sci_issues&pid=0327-9545&lng=en&nrm=iso},
volume = {36},
year = {2012}
}
TY - JOUR
ID - 989665
AU - Vařecha, Miroslav - Páclová, Daniela - Procházková, Jiřina - Matula, Pavel - Cmarko, Dušan - Kozubek, Michal
PY - 2012
TI - Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I
JF - BIOCELL
VL - 36
IS - 3
SP - 121-126
EP - 121-126
PB - National Council of Scientific and Techn
SN - 03279545
N1 - Accepted.
KW - superoxide;fluorescence microscopy;image analysis;mitochondria;telomerase
UR - http://www.scielo.org.ar/scielo.php?script=sci_issues&pid=0327-9545&lng=en&nrm=iso
N2 - Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Our results show that tumoral telomerase-positive (TP) AIF knockdown cell lines display significant increase in superoxide production in comparison to control cells, while a non-tumoral cell line and tumoral telomerase-negative cell lines with alternative lengthening of telomeres (ALT) show a decrease in superoxide production. According to these results, we can conclude that AIF knockdown disrupts function of complex I and therefore increases the superoxide production in mitochondria. The distinct effect of AIF depletion in various cell lines could result from recently discovered activity of telomerase in mitochondria of TP cancer cells, but this hypothesis needs further investigation.
ER -
VAŘECHA, Miroslav, Daniela PÁCLOVÁ, Jiřina PROCHÁZKOVÁ, Pavel MATULA, Dušan CMARKO and Michal KOZUBEK. Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I. \textit{BIOCELL}. Argentina: National Council of Scientific and Techn, 2012, vol.~36, No~3, p.~121-126. ISSN~0327-9545.
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