a 2012

Cloning and characterization of novel jacalin-related lectin from Aspergillus fumigatus

KOMÁREK, Jan, Josef HOUSER, Anne IMBERTY and Michaela WIMMEROVÁ

Basic information

Original name

Cloning and characterization of novel jacalin-related lectin from Aspergillus fumigatus

Authors

KOMÁREK, Jan (203 Czech Republic), Josef HOUSER (203 Czech Republic), Anne IMBERTY (250 France) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

XXIII. biochemický sjezd, 2012

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/12:00057533

Organization unit

Faculty of Science

ISBN

978-80-86313-34-4

Keywords (in Czech)

Aspergillus fumigatus; lektiny

Keywords in English

Aspergillus fumigatus; lectins
Změněno: 27/8/2012 08:19, Mgr. Jan Komárek, Ph.D.

Abstract

V originále

Lectins are proteins or glycoproteins of non-immune origin which recognize and bind reversibly to diverse sugar structures. They are involved in recognition events in a variety of biological processes, such as regulation of cell cycle, targeting of cells, host-pathogen interactions, plant defense and cell differentiation. Jacalin-related proteins (JRPs) represent a family of lectins that are structurally related to jacalin, a well-known plant seed lectin isolated from jack fruit. JRPs are widespread among plants, while only few members have been found in fungi so far. Despite high sequence similarity among jacalin-related proteins, they differ from each other with respect to carbohydrate-binding preferences. Aspergillus fumigatus is an ubiquitous saprophytic mold and opportunistic human pathogen responsible for severe infections in cystic fibrosis and lung transplant patients. Inhalation of the spores from the environment can result in invasive fungal infections and represents a serious threat for individuals with compromised immunity. In A. fumigatus, lectins are supposed to provide a possible mechanism of attachment of conidia to host tissues and could be an important virulence factor. The project is focused on cloning and characterization of a hypothetical lectin AFL4 from ascomycete A. fumigatus which shares a sequence similarity with JRPs. The afl4 gene was amplified, cloned into a plasmid vector and expressed in bacterial expression system. AFL4 is currently experimentally studied and should be characterized from structure-function point of view.

Links

CZ.1.05/1.1.00/02.0068, interní kód MU
Name: CEITEC - středoevropský technologický institut (Acronym: CEITEC)
Investor: Ministry of Education, Youth and Sports of the CR, CEITEC - Central European Institute of Technology, 1.1 European Centres of Excellence
GA303/09/1168, research and development project
Name: Lektiny z lidských patogenů - struktura, funkce, inženýrství
Investor: Czech Science Foundation, Lectins from human pathogens - structure, function, engineering
GD301/09/H004, research and development project
Name: Molekulární a strukturní biologie vybraných cytostatik. Od mechanistických studií k chemoterapii rakoviny
Investor: Czech Science Foundation