LEE, Lan-Ying, Fu-Hui WU, Chen-Tran HSU, Shu-Chen SHEN, Hsuan-Yu YEH, De-Chih LIAO, Mei-Jane FANG, Nien-Tze LIU, Yu-Chen YEN, Ladislav DOKLÁDAL, Eva SÝKOROVÁ, Stanton B. GELVIN and Choun-Sea LIN. Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. Plant Cell. UNITED STATES: AMER SOC PLANT BIOLOGISTS, vol. 24, No 5, p. 1746-1759. ISSN 1040-4651. doi:10.1105/tpc.112.097998. 2012.
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Basic information
Original name Screening a cDNA Library for Protein-Protein Interactions Directly in Planta
Authors LEE, Lan-Ying (158 Taiwan), Fu-Hui WU (158 Taiwan), Chen-Tran HSU (158 Taiwan), Shu-Chen SHEN (158 Taiwan), Hsuan-Yu YEH (158 Taiwan), De-Chih LIAO (158 Taiwan), Mei-Jane FANG (158 Taiwan), Nien-Tze LIU (158 Taiwan), Yu-Chen YEN (158 Taiwan), Ladislav DOKLÁDAL (203 Czech Republic, belonging to the institution), Eva SÝKOROVÁ (203 Czech Republic, guarantor, belonging to the institution), Stanton B. GELVIN (840 United States of America) and Choun-Sea LIN (158 Taiwan).
Edition Plant Cell, UNITED STATES, AMER SOC PLANT BIOLOGISTS, 2012, 1040-4651.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 9.251
RIV identification code RIV/00216224:14740/12:00057108
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1105/tpc.112.097998
UT WoS 000306105400008
Keywords in English BIMOLECULAR FLUORESCENCE COMPLEMENTATION; STRAND TELOMERIC DNA; BINDING-PROTEIN; NUCLEAR IMPORT; IN-PLANTA; T-DNA; VIRD2 PROTEIN; CELLS; TRANSFORMATION; VISUALIZATION
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 23/3/2013 06:52.
Abstract
Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. T-DNA insertions in the genes represented by some cDNAs revealed five novel Arabidopsis proteins important for Agrobacterium-mediated plant transformation. We also used this cDNA library to confirm VirE2-interacting proteins in orchid (Phalaenopsis amabilis) flowers. Thus, this technology can be applied to several plant species.
Links
IAA500040801, research and development projectName: Telomery a telomerázy: posun od molekulárně-biologického ke strukturně-biologickému přístupu.
Investor: Academy of Sciences of the Czech Republic, Telomeres and telomerase: transition from molecular to structural biology approach
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