LEE, Lan-Ying, Fu-Hui WU, Chen-Tran HSU, Shu-Chen SHEN, Hsuan-Yu YEH, De-Chih LIAO, Mei-Jane FANG, Nien-Tze LIU, Yu-Chen YEN, Ladislav DOKLÁDAL, Eva SÝKOROVÁ, Stanton B. GELVIN and Choun-Sea LIN. Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. Plant Cell. UNITED STATES: AMER SOC PLANT BIOLOGISTS, 2012, vol. 24, No 5, p. 1746-1759. ISSN 1040-4651. Available from: https://dx.doi.org/10.1105/tpc.112.097998. |
Other formats:
BibTeX
LaTeX
RIS
@article{990393, author = {Lee, LanandYing and Wu, FuandHui and Hsu, ChenandTran and Shen, ShuandChen and Yeh, HsuanandYu and Liao, DeandChih and Fang, MeiandJane and Liu, NienandTze and Yen, YuandChen and Dokládal, Ladislav and Sýkorová, Eva and Gelvin, Stanton B. and Lin, ChounandSea}, article_location = {UNITED STATES}, article_number = {5}, doi = {http://dx.doi.org/10.1105/tpc.112.097998}, keywords = {BIMOLECULAR FLUORESCENCE COMPLEMENTATION; STRAND TELOMERIC DNA; BINDING-PROTEIN; NUCLEAR IMPORT; IN-PLANTA; T-DNA; VIRD2 PROTEIN; CELLS; TRANSFORMATION; VISUALIZATION}, language = {eng}, issn = {1040-4651}, journal = {Plant Cell}, title = {Screening a cDNA Library for Protein-Protein Interactions Directly in Planta}, url = {http://www.plantcell.org/content/24/5/1746.abstract}, volume = {24}, year = {2012} }
TY - JOUR ID - 990393 AU - Lee, Lan-Ying - Wu, Fu-Hui - Hsu, Chen-Tran - Shen, Shu-Chen - Yeh, Hsuan-Yu - Liao, De-Chih - Fang, Mei-Jane - Liu, Nien-Tze - Yen, Yu-Chen - Dokládal, Ladislav - Sýkorová, Eva - Gelvin, Stanton B. - Lin, Choun-Sea PY - 2012 TI - Screening a cDNA Library for Protein-Protein Interactions Directly in Planta JF - Plant Cell VL - 24 IS - 5 SP - 1746-1759 EP - 1746-1759 PB - AMER SOC PLANT BIOLOGISTS SN - 10404651 KW - BIMOLECULAR FLUORESCENCE COMPLEMENTATION KW - STRAND TELOMERIC DNA KW - BINDING-PROTEIN KW - NUCLEAR IMPORT KW - IN-PLANTA KW - T-DNA KW - VIRD2 PROTEIN KW - CELLS KW - TRANSFORMATION KW - VISUALIZATION UR - http://www.plantcell.org/content/24/5/1746.abstract L2 - http://www.plantcell.org/content/24/5/1746.abstract N2 - Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. T-DNA insertions in the genes represented by some cDNAs revealed five novel Arabidopsis proteins important for Agrobacterium-mediated plant transformation. We also used this cDNA library to confirm VirE2-interacting proteins in orchid (Phalaenopsis amabilis) flowers. Thus, this technology can be applied to several plant species. ER -
LEE, Lan-Ying, Fu-Hui WU, Chen-Tran HSU, Shu-Chen SHEN, Hsuan-Yu YEH, De-Chih LIAO, Mei-Jane FANG, Nien-Tze LIU, Yu-Chen YEN, Ladislav DOKLÁDAL, Eva SÝKOROVÁ, Stanton B. GELVIN and Choun-Sea LIN. Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. \textit{Plant Cell}. UNITED STATES: AMER SOC PLANT BIOLOGISTS, 2012, vol.~24, No~5, p.~1746-1759. ISSN~1040-4651. Available from: https://dx.doi.org/10.1105/tpc.112.097998.
|