Electrochemical Behaviour of Apoferritin Encapsulating of
Silver(I) Ions and Its Application for Treatment of
Staphylococcus aureus

J 2012

Electrochemical Behaviour of Apoferritin Encapsulating of Silver(I) Ions and Its Application for Treatment of Staphylococcus aureus

DOSPIVOVÁ, Dana, David HYNEK, Pavel KOPEL, Andrea BEZDĚKOVÁ, Jiří SOCHOR et. al.

Basic information

Original name

Electrochemical Behaviour of Apoferritin Encapsulating of Silver(I) Ions and Its Application for Treatment of Staphylococcus aureus

Authors

DOSPIVOVÁ, Dana (203 Czech Republic), David HYNEK (203 Czech Republic), Pavel KOPEL (203 Czech Republic), Andrea BEZDĚKOVÁ (203 Czech Republic), Jiří SOCHOR (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution), Jaromír HUBÁLEK (203 Czech Republic), Petr BABULA (203 Czech Republic), Ivo PROVAZNÍK (203 Czech Republic), Radimír VRBA (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)

Edition

International Journal of Electrochemical Science, 2012, 1452-3981

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

Serbia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.729 in 2011

RIV identification code

RIV/00216224:14310/12:00060702

Organization unit

Faculty of Science

UT WoS

000306399700051

Keywords in English

electrochemical detection; silver; nanomaterial; nanomedicine

Abstract

V originále

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for several difficult-to-treat infections in humans. Therefore, it is not surprising that other ways how to treat these bacteria are looked for. Silver(I) ions and silver nanoparticles exhibited the highest antimicrobial activity against MRSA but their transporting to the place of needs and in situ determination is an issue. The aim of this work was electrochemical determination of silver(I) ions using four types of modified carbon paste electrodes (CPEs) with different content of carbon nanoparticles. CPE made from expanded carbon was the most sensitive one. Therefore, we optimized the experimental conditions as time of accumulation 60 s, deposition potential 0.5 V and 0.2 M acetate buffer, pH = 5.0 to obtain detection limit (3 S/N) of 5 nM for silver(I) ions. Further, we studied the encapsulation of silver(I) ions into apoferritin as a possible way for transportation of these ions. Primarily we optimized the encapsulation conditions to prepare the most stable complex, which was subsequently utilized for treatment of S. aureus. Based on the results obtained it can be concluded that silver(I) ions remain enclosed in the apoferritin structure until decomposition of apoferritin by bacterial enzymatic apparatus occurs.

Citovat

DOSPIVOVÁ, Dana, David HYNEK, Pavel KOPEL, Andrea BEZDĚKOVÁ, Jiří SOCHOR, Soňa KŘÍŽKOVÁ, Vojtěch ADAM, Libuše TRNKOVÁ, Jaromír HUBÁLEK, Petr BABULA, Ivo PROVAZNÍK, Radimír VRBA and René KIZEK. Electrochemical Behaviour of Apoferritin Encapsulating of Silver(I) Ions and Its Application for Treatment of Staphylococcus aureus. International Journal of Electrochemical Science. 2012, vol. 7, No 7, p. 6378-6395. ISSN 1452-3981.

@article{990416,
   author = {Dospivová, Dana and Hynek, David and Kopel, Pavel and Bezděková, Andrea and Sochor, Jiří and Křížková, Soňa and Adam, Vojtěch and Trnková, Libuše and Hubálek, Jaromír and Babula, Petr and Provazník, Ivo and Vrba, Radimír and Kizek, René},
   article_number = {7},
   keywords = {electrochemical detection; silver; nanomaterial; nanomedicine},
   language = {eng},
   issn = {1452-3981},
   journal = {International Journal of Electrochemical Science},
   title = {Electrochemical Behaviour of Apoferritin Encapsulating of Silver(I) Ions and Its Application for Treatment of Staphylococcus aureus},
   volume = {7},
   year = {2012}
}

TY  - JOUR
ID  - 990416
AU  - Dospivová, Dana - Hynek, David - Kopel, Pavel - Bezděková, Andrea - Sochor, Jiří - Křížková, Soňa - Adam, Vojtěch - Trnková, Libuše - Hubálek, Jaromír - Babula, Petr - Provazník, Ivo - Vrba, Radimír - Kizek, René
PY  - 2012
TI  - Electrochemical Behaviour of Apoferritin Encapsulating of Silver(I) Ions and Its Application for Treatment of Staphylococcus aureus
JF  - International Journal of Electrochemical Science
VL  - 7
IS  - 7
SP  - 6378-6395
EP  - 6378-6395
SN  - 14523981
KW  - electrochemical detection
KW  - silver
KW  - nanomaterial
KW  - nanomedicine
N2  - Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for several difficult-to-treat infections in humans. Therefore, it is not surprising that other ways how to treat these bacteria are looked for. Silver(I) ions and silver nanoparticles exhibited the highest antimicrobial activity against MRSA but their transporting to the place of needs and in situ determination is an issue. The aim of this work was electrochemical determination of silver(I) ions using four types of modified carbon paste electrodes (CPEs) with different content of carbon nanoparticles. CPE made from expanded carbon was the most sensitive one. Therefore, we optimized the experimental conditions as time of accumulation 60 s, deposition potential 0.5 V and 0.2 M acetate buffer, pH = 5.0 to obtain detection limit (3 S/N) of 5 nM for silver(I) ions. Further, we studied the encapsulation of silver(I) ions into apoferritin as a possible way for transportation of these ions. Primarily we optimized the encapsulation conditions to prepare the most stable complex, which was subsequently utilized for treatment of S. aureus. Based on the results obtained it can be concluded that silver(I) ions remain enclosed in the apoferritin structure until decomposition of apoferritin by bacterial enzymatic apparatus occurs.
ER  -

DOSPIVOVÁ, Dana, David HYNEK, Pavel KOPEL, Andrea BEZDĚKOVÁ, Jiří SOCHOR, Soňa KŘÍŽKOVÁ, Vojtěch ADAM, Libuše TRNKOVÁ, Jaromír HUBÁLEK, Petr BABULA, Ivo PROVAZNÍK, Radimír VRBA and René KIZEK. Electrochemical Behaviour of Apoferritin Encapsulating of Silver(I) Ions and Its Application for Treatment of Staphylococcus aureus. \textit{International Journal of Electrochemical Science}. 2012, vol.~7, No~7, p.~6378-6395. ISSN~1452-3981.

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