Single-strand Conformation Polymorphism Studied by Elimination
Voltammetry - New Challenge in DNA Sensing

D 2012

Single-strand Conformation Polymorphism Studied by Elimination Voltammetry - New Challenge in DNA Sensing

TRNKOVÁ, Libuše, František JELEN, Vojtěch ADAM and René KIZEK

Basic information

Original name

Single-strand Conformation Polymorphism Studied by Elimination Voltammetry - New Challenge in DNA Sensing

Authors

TRNKOVÁ, Libuše, František JELEN, Vojtěch ADAM and René KIZEK

Edition

Brno, 45th Heyrovsky Discussion Electrochemistry of Biopolymers and Bioactive Compounds, Book of Abstract, p. 38-38, 2012

Publisher

CEITEC; J. Heyrovský institue of Physical Chemistry of the AS CR, v. v. i.;BFU Brno

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10403 Physical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

Organization unit

Faculty of Science

ISBN

978-80-87541-03-6

Keywords in English

SSCP; single-strand conformation polymorphism;cytosine;i-motif;LSV;EVLS;ODN

Změněno: 17/1/2013 09:51, Mgr. Sylvie Dohnalíková

Abstract

V originále

Single-strand conformation polymorphism (SSCP) is defined as a conformational difference of single-stranded nucleotide sequences. For example, the cytosine (C)-rich single-stranded DNA can adopt i-motif structure consisting of intercalated hemiprotonated C–C+ base pairing [1,2]. Using linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) [3,4] we studied the effect of pH, the number and sequence of C in homo and hetero-ODN chains on the reduction signals on mercury electrodes in buffered and non-buffered solutions. The EVLS procedure in connection with adsorptive stripping techniques (AdS) was found to be capable of revealing the changed ODN structure on a mercury electrode surface and reflects the single-strand chain polymorphism. For this purpose the EVLS function E4 conserving the diffusion current and eliminating the kinetic and charging current components was used. Compared to common voltammetric methods, the EVLS E4 function is capable of providing 7-15 fold sensitivity and a better resolution of redox signals of the nucleobases in the ODN chain [5]. This benefit is particularly pronounced in the case of an adsorbed substance yielding a well-developed elimination signal (peak-counterpeak) which does not require a baseline correction. Changes in the nucleotide sequence, owing to a polymorphism, are expected to alter the secondary structure of the ODN molecules resulting in a shift in their redox and adsorption properties. Although SSCP analysis is primarily associated with the electrophoretic methods and the polymerase chain reaction [6] our AdS EVLS can quickly identify different conformers in solutions and on the electrode surface in dependence on experimental conditions such as temperature, pH, ionic strength and buffer components.

Links

MUNI/A/0992/2009, interní kód MU

Name: Vývoj metodologie a instrumentace analytické chemie pro studium složení a vlastností přírodních a syntetických materiálů (Acronym: MIA)

Investor: Masaryk University, Category A

Citovat

TRNKOVÁ, Libuše, František JELEN, Vojtěch ADAM and René KIZEK. Single-strand Conformation Polymorphism Studied by Elimination Voltammetry - New Challenge in DNA Sensing. In Fojta Miroslav;Navrátil Tomáš;Pivoňková Hana. 45th Heyrovsky Discussion Electrochemistry of Biopolymers and Bioactive Compounds, Book of Abstract. Brno: CEITEC; J. Heyrovský institue of Physical Chemistry of the AS CR, v. v. i.;BFU Brno, 2012, p. 38. ISBN 978-80-87541-03-6.

@inproceedings{990435,
   author = {Trnková, Libuše and Jelen, František and Adam, Vojtěch and Kizek, René},
   address = {Brno},
   booktitle = {45th Heyrovsky Discussion Electrochemistry of Biopolymers and Bioactive Compounds, Book of Abstract},
   editor = {Fojta Miroslav;Navrátil Tomáš;Pivoňková Hana},
   keywords = {SSCP; single-strand conformation polymorphism;cytosine;i-motif;LSV;EVLS;ODN},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-87541-03-6},
   pages = {38-38},
   publisher = {CEITEC; J. Heyrovský institue of Physical Chemistry of the AS CR, v. v. i.;BFU Brno},
   title = {Single-strand Conformation Polymorphism Studied by Elimination Voltammetry - New Challenge in DNA Sensing},
   year = {2012}
}

TY  - JOUR
ID  - 990435
AU  - Trnková, Libuše - Jelen, František - Adam, Vojtěch - Kizek, René
PY  - 2012
TI  - Single-strand Conformation Polymorphism Studied by Elimination Voltammetry - New Challenge in DNA Sensing
PB  - CEITEC; J. Heyrovský institue of Physical Chemistry of the AS CR, v. v. i.;BFU Brno
CY  - Brno
SN  - 9788087541036
KW  - SSCP
KW  - single-strand conformation polymorphism;cytosine;i-motif;LSV;EVLS;ODN
N2  - Single-strand conformation polymorphism (SSCP) is defined as a conformational difference of single-stranded nucleotide sequences. For example, the cytosine (C)-rich single-stranded DNA can adopt i-motif structure consisting of intercalated hemiprotonated C–C+ base pairing [1,2]. Using linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) [3,4] we studied the effect of pH, the number and sequence of C in homo and hetero-ODN chains on the reduction signals on mercury electrodes in buffered and non-buffered solutions. The EVLS procedure in connection with adsorptive stripping techniques (AdS) was found to be capable of revealing the changed ODN structure on a mercury electrode surface and reflects the single-strand chain polymorphism. For this purpose the EVLS function E4 conserving the diffusion current and eliminating the kinetic and charging current components was used. Compared to common voltammetric methods, the EVLS E4 function is capable of providing 7-15 fold sensitivity and a better resolution of redox signals of the nucleobases in the ODN chain [5]. This benefit is particularly pronounced in the case of an adsorbed substance yielding a well-developed elimination signal (peak-counterpeak) which does not require a baseline correction. Changes in the nucleotide sequence, owing to a polymorphism, are expected to alter the secondary structure of the ODN molecules resulting in a shift in their redox and adsorption properties. Although SSCP analysis is primarily associated with the electrophoretic methods and the polymerase chain reaction [6] our AdS EVLS can quickly identify different conformers in solutions and on the electrode surface in dependence on experimental conditions such as temperature, pH, ionic strength and buffer components.
ER  -

TRNKOVÁ, Libuše, František JELEN, Vojtěch ADAM and René KIZEK. Single-strand Conformation Polymorphism Studied by Elimination Voltammetry - New Challenge in DNA Sensing. In Fojta Miroslav;Navrátil Tomáš;Pivoňková Hana. \textit{45th Heyrovsky Discussion Electrochemistry of Biopolymers and Bioactive Compounds, Book of Abstract}. Brno: CEITEC; J. Heyrovský institue of Physical Chemistry of the AS CR, v. v. i.;BFU Brno, 2012, p.~38. ISBN~978-80-87541-03-6.

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